Immunocytological localization of two plant fatty acid desaturases in the endoplasmic reticulum
Immunocytological localization of two plant fatty acid desaturases in the endoplasmic reticulum
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DOI：
10.1016/s0014-5793(01)02315-8
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发表时间：
2001-04-06
期刊：
影响因子：
3.5
通讯作者：
Mullen, RT
中科院分区：
文献类型：
--
作者：
Dyer, JM;Mullen, RT
通过免疫荧光显微镜对瞬时转化不同表位标记形式的烟草悬浮细胞的Fad2和Fad3两种整合膜结合脂肪酸脱氢酶(FADS)的亚细胞定位进行了研究。Myc或血凝素标记的Fad2和Fad3都定位于内质网(ER)的同一区域，与ER腔蛋白calretictin共定位证明。差异渗透实验结果表明，标记表位的Fad2和Fad3的N末端暴露在ER膜的胞液侧，这些数据定义了植物脱饱和酶在ER膜中的亚细胞位置和拓扑方向。(C)2001年，埃尔塞维尔科学公司代表欧洲生物化学学会联合会出版。
The subcellular location of two integral membrane-bound fatty acid desaturases (Fads), Fad2 and Fad3, was elucidated by immunofluorescence microscopic analyses of tobacco suspension cells transiently transformed with different epitope-tagged versions of the enzymes. Both myc- or hemagglutinin-tagged Fad2 and Fad3 localized to the same region of the endoplasmic reticulum (ER), as evidenced by their co-localization with the ER lumenal protein calreticulin, Results from differential permeabilization experiments revealed that the N-termini of both epitope-tagged Fad2 and Fad3 were exposed on the cytosolic side of ER membranes, These data define the subcellular location and topological orientation of plant desaturases in ER membranes. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.