The effects of pancreatic polypeptide on locomotor activity and food intake in mice
The effects of pancreatic polypeptide on locomotor activity and food intake in mice
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DOI：
10.1038/ijo.2008.160
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发表时间：
2008-11-01
影响因子：
4.9
通讯作者：
Bloom, S. R.
中科院分区：
文献类型：
--
作者：
Liu, Y-L;Semjonous, N. M.;Bloom, S. R.
胰多肽（PP）已被证明可以抑制啮齿动物和人类的食物摄入。急性外周给予PP增加肥胖小鼠的耗氧量。为了进一步研究PP在调节能量平衡中的功能，我们使用24室综合实验动物监测系统检测了其对小鼠自发运动活动和摄食的影响。腹膜内（i. p.）使用光束技术测量PP给药对自发运动活动的影响。PP剂量依赖性地抑制累积摄食量。在30、100和300 nmol kg（-1）PP剂量下，抑制作用分别在6、17和36 h内保持显著。在10 nmol kg（-1）时，PP在给药后4 h显著增加自发活动（累积射束中断），而不影响摄食量，在30 nmol kg（-1）时，与对照组相比，PP在注射后5 h增加自发活动79%。然而，在100和300 nmol kg（-1），PP对自发活动没有显着影响。这项研究首次表明，PP增加小鼠的自发运动活动。
Pancreatic polypeptide (PP) has been shown to inhibit food intake in both rodents and humans. Acute peripheral administration of PP increases oxygen consumption in obese mice. To further investigate the function of PP in the modulation of energy balance, we examined its effects on spontaneous locomotor activity and food intake in mice by using a 24-chamber Comprehensive Laboratory Animal Monitoring System. Effects of intraperitoneal (i.p.) administration of PP on spontaneous locomotor activity were measured using the optical beam technique. Administration of PP dose dependently inhibited cumulative food intake. The inhibition remained significant for up to 6, 17 and 36 h at doses of 30, 100 and 300 nmol kg(-1) PP, respectively. At 10 nmol kg(-1), PP increased locomotor activity (cumulative beam breaks) significantly for 4 h following administration without affecting food intake and at 30 nmol kg(-1), PP increased locomotor activity by 79% compared with the controls for 5 h post injection. However, at 100 and 300 nmol kg(-1), PP had no significant effect on locomotor activity. This study shows for the first time that PP increases spontaneous locomotor activity in mice.