In vivo gene transfer using pDNA/chitosan/chondroitin sulfate ternary complexes: influence of chondroitin sulfate on the stability of freeze‐dried complexes and transgene expression in vivo
In vivo gene transfer using pDNA/chitosan/chondroitin sulfate ternary complexes: influence of chondroitin sulfate on the stability of freeze‐dried complexes and transgene expression in vivo
复制标题
DOI：
10.1002/jgm.2694
复制
发表时间：
2013-02
期刊：
影响因子：
--
通讯作者：
K. Hagiwara;S. Kishimoto;M. Ishihara;Y. Koyama;O. Mazda;Toshinori Sato
中科院分区：
文献类型：
--
作者：
K. Hagiwara;S. Kishimoto;M. Ishihara;Y. Koyama;O. Mazda;Toshinori Sato
壳聚糖被认为是一种很有前途的非病毒载体。然而，仍然存在一些问题，如相对较低的转染率和生理条件下的不稳定性。我们在体外实验中证明了硫酸软骨素(CS)包衣提高了质粒DNA(PDNA)/壳聚糖复合体的转染率和物理化学稳定性。本研究考察了壳聚糖包被pDNA/壳聚糖复合体对冻干复水过程稳定性和体内基因表达的影响。
Chitosan has been investigated as a promising nonviral vector. However, several problems still remain, such as a relatively low transfection efficiency and instability under physiological conditions. We previously demonstrated that a chondroitin sulfate (CS) coating enhanced the transfection efficiency and physicochemical stability of plasmid DNA (pDNA)/chitosan complexes in vitro. In the present study, the effects of coating pDNA/chitosan complexes with CS on the stability in freeze–dry rehydration processes and gene expression in vivo were investigated.