Effects of subcutaneous administration of glutamate on pain, sensitization and vasomotor responses in healthy men and women
Effects of subcutaneous administration of glutamate on pain, sensitization and vasomotor responses in healthy men and women
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DOI：
10.1016/j.pain.2006.06.015
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发表时间：
2006-10-01
期刊：
影响因子：
7.4
通讯作者：
Arendt-Nielsen, Lars
中科院分区：
文献类型：
--
作者：
Gazerani, Parisa;Wang, Kelun;Arendt-Nielsen, Lars
本研究旨在探讨(1)皮下注射谷氨酸是否会引起人类的疼痛、致敏和血管舒缩反应，以及(2)这些反应是否存在性别差异。包括30名健康志愿者（男性15岁，女性15岁）。每名受试者在前额皮肤皮下注射4次皮下注射（0.1 ml；谷氨酸100、10、1 mM和0.9%等渗盐水），分2次注射，间隔1周。疼痛强度评估（VAS）、质量、分布；针刺痛觉过敏面积；注射部位压痛阈值（PPT）；在预定时间点测量皮肤表面温度和局部血流量。谷氨酸浓度最高时，男性和女性的疼痛强度最高，疼痛持续时间最长，vas时间曲线下的疼痛面积最大（P < 0.001），但女性的反应大于男性（P < 0.05）。当谷氨酸浓度最高时，面部疼痛区域最大（P < 0.001），女性的疼痛区域比男性大（P=0.024）。谷氨酸100 mM组痛觉针刺区面积最大（P < 0.001），女性痛觉针刺区面积大于男性（P < 0.001）。皮下注射谷氨酸后发现了浓度依赖性的局部血管舒缩反应，但这种效果没有性别差异。谷氨酸100 mM显著降低PPT值（P < 0.001），无性别差异。本研究首次证明，皮下注射谷氨酸可引起人类志愿者的疼痛、血管舒缩反应和针刺痛觉过敏，并且其中一些反应存在性别差异。(c) 2006国际疼痛研究协会。Elsevier B.V.版权所有。
The present study aimed to investigate if (1) subcutaneous injection of glutamate induces pain, sensitization and vasomotor responses in humans and (2) if sex differences exist in these responses. Thirty healthy volunteers (men-15 and women-15) were included. Each subject received four subcutaneous injections (0.1 ml; glutamate 100, 10, 1 mM and isotonic saline 0.9%) into the forehead skin in two sessions separated by one week. Assessments of pain intensity (VAS), quality, distribution; area of pinprick hyperalgesia; pressure pain threshold (PPT) at the injection site; surface skin temperature and local blood flow were performed at predetermined time points. The highest concentration of glutamate evoked the highest pain intensity, the longest duration of pain and the largest pain area under the VAS-time curve (P < 0.001) in both men and women, although responses in women were larger than in men (P < 0.05). The face-chart pain area was the largest for the highest concentration of glutamate (P < 0.001) and women drew a larger pain area than men (P=0.024). The area of pinprick hyperalgesia was the largest for glutamate 100 mM (P < 0.001) and women indicated a larger area than men (P < 0.001). Concentration-dependent local vasomotor responses were found following the subcutaneous injection of glutamate but there was no sex difference in this effect. Glutamate 100 mM significantly reduced the PPT values (P < 0.001) without sex-related differences. The present study demonstrates for the first time that subcutaneous injection of glutamate evokes pain, vasomotor responses and pinprick hyperalgesia in human volunteers and that there are sex-related differences in some of these responses. (c) 2006 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.