FINE-STRUCTURAL OBSERVATIONS ON FORM AND DISTRIBUTION OF NUAGE IN GERM-CELLS OF RAT
FINE-STRUCTURAL OBSERVATIONS ON FORM AND DISTRIBUTION OF NUAGE IN GERM-CELLS OF RAT
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DOI：
10.1002/ar.1091780406
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发表时间：
1974-01-01
影响因子：
--
通讯作者：
EDDY, EM
中科院分区：
文献类型：
--
作者：
EDDY, EM
人们对大鼠生殖细胞的精细结构水平进行了研究，从它们存在于胚胎肠道上皮中的时间开始，直到接近成年配子产生的时间为止。在生命周期的这一时期，生殖细胞中致密纤维材料（nuage）的形式和位置受到了特别关注。 nuage 要么作为细胞质中的小离散体存在，要么作为位于线粒体簇间隙中的“胶结材料”存在。它存在于肠上皮的原始生殖细胞、中性性腺的生殖细胞以及性分化的胎儿、新生儿和成年大鼠性腺的生殖细胞中。有时它与核孔有关。因为它存在于雄性和雌性大鼠生殖细胞的大部分生命周期中，并且以前的研究人员已经在各种其他哺乳动物中观察到它，所以这里表明它是哺乳动物生殖细胞的特征性形态特征。此外，nuage在形式和分布上与昆虫的“极性颗粒”和两栖动物的“生殖质”有相当大的相似性，这表明它们在确定这些动物的生殖细胞中发挥着作用。考虑到 nuage 在哺乳动物中可能发挥类似作用的可能性。
Germ cells of the rat have been studied at the fine structural level from the time they are present in the epithelium of the embryonic gut until near the time of production of mature gametes in the adult. Particular attention has been paid to the form and location of dense, fibrous material (nuage) in the germ cells during that period of the life cycle. The nuage either exists as small discrete bodies in the cytoplasm or as “cementing material” situated in the interstices of mitochondrial clusters. It is present in primordial germ cells in the gut epithelium, in germ cells in indifferent gonads and in germ cells in sexually differentiated fetal, neonatal and adult rat gonads. It is sometimes associated with nuclear pores. Because it is present throughout much of the life cycle of the germ cells of male and female rats and it has been observed in various other mammals by previous workers, it is suggested here that it is a characteristic morphological feature of mammalian germ cells. In addition, there is considerable similarity in form and distribution of the nuage to the “polar granules” in insects and the “germinal plasm” in amphibians which are suggested to play a role in the determination of germ cells in these animals. The possibility that nuage may play a comparable role in mammals is considered.