Expression, Localization, and Binding Activity of the Ezrin/Radixin/Moesin Proteins in the Mouse Testis
Expression, Localization, and Binding Activity of the Ezrin/Radixin/Moesin Proteins in the Mouse Testis
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DOI：
10.1369/jhc.2008.952440
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发表时间：
2009-04
影响因子：
3.2
通讯作者：
T. Wakayama;Hiroki Nakata;Miho Kurobo;Y. Sai;S. Iseki
中科院分区：
文献类型：
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作者：
T. Wakayama;Hiroki Nakata;Miho Kurobo;Y. Sai;S. Iseki
埃兹蛋白、根蛋白和膜突蛋白（ERM）蛋白代表将跨膜蛋白连接到细胞骨架的衔接蛋白家族。生精上皮的细胞组成、位置和形状发生了广泛的变化，暗示了膜-细胞骨架相互作用的作用。然而，目前还不清楚ERM蛋白是否在睾丸中表达并发挥重要作用。在本研究中，我们研究了ERM蛋白的时空表达在小鼠睾丸的蛋白质免疫印迹和免疫组织化学。Ezrin免疫反应性表现在产后5周至成年期的第15和16步精子细胞的细胞质中，而Radixin免疫反应性表现在产后1周至2周的支持细胞的顶端细胞质中。在任何年龄段均未检测到膜突蛋白的免疫反应性。免疫沉淀表明，ezrin绑定到细胞骨架组分肌动蛋白，而radixin绑定到肌动蛋白和微管蛋白。在已知与ERM蛋白相互作用的跨膜蛋白中，只有囊性纤维化跨膜传导调节因子（一种氯离子转运蛋白）与细长精子细胞中的ezrin结合。这些结果表明，ezrin参与精子发生，而radixin参与支持细胞的成熟，通过与不同的膜蛋白和细胞骨架成分的相互作用。
The ezrin, radixin, and moesin (ERM) proteins represent a family of adaptor proteins linking transmembrane proteins to the cytoskeleton. The seminiferous epithelium undergoes extensive changes in cellular composition, location, and shape, implicating roles of the membrane–cytoskeleton interaction. It remains unknown, however, whether the ERM proteins are expressed and play significant roles in the testis. In the present study, we examined the spatiotemporal expression of ERM proteins in the mouse testis by Western blotting and immunohistochemistry. Ezrin immunoreactivity was demonstrated in the cytoplasm of steps 15 and 16 spermatids from 5 weeks postpartum through adulthood, whereas radixin immunoreactivity was in the apical cytoplasm of Sertoli cells from 1 week through 2 weeks postpartum. No immunoreactivity for moesin was detected at any age. Immuno-precipitation demonstrated that ezrin was bound to the cytoskeletal component actin, whereas radixin was bound to both actin and tubulin. Of the transmembrane proteins known to interact with ERM proteins, only cystic fibrosis transmembrane conductance regulator, a chloride transporter, was bound to ezrin in elongated spermatids. These results suggest that ezrin is involved in spermiogenesis whereas radixin is involved in the maturation of Sertoli cells, through interaction with different sets of membrane proteins and cyto-skeletal components.