Formation kinetics and fractal characteristics of regenerated silk fibroin alcogel developed from nanofibrillar network
Formation kinetics and fractal characteristics of regenerated silk fibroin alcogel developed from nanofibrillar network
复制标题
纳米纤丝网络再生丝素蛋白醇凝胶的形成动力学和分形特征
DOI：
10.1039/b913510c
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发表时间：
2010-01-01
期刊：
影响因子：
3.4
通讯作者：
Shao, Zhengzhong
中科院分区：
文献类型：
--
作者：
Gong, Zuguang;Yang, Yuhong;Shao, Zhengzhong
通过TEM、AFM和共聚焦激光扫描显微镜（CLSM）观察RSF醇凝胶的形貌，并通过硫磺素T荧光和流变学方法研究其动力学，阐明了RSF醇凝胶的形成机理。一个有趣的双重结构-纳米纤维网络的纳米尺度和絮状网络的微观尺度上观察到的RSF醇凝胶内。基于我们的动力学研究，发现的分支纤维状聚集体的分形维数为2.4-2.8取决于RSF浓度。同时，通过对RSF醇凝胶力学性能的标度分析，发现絮体连接网络的分形维数为2.2。此外，我们的研究结果表明，RSF的原纤化是由RSF和共溶剂之间的相互作用的急剧变化引发的，并且凝胶化动力学可以通过调节乙醇的浓度以及RSF来调节。这种生物高分子凝胶的可控的双重结构和动力学特性使其在纳米技术中具有潜在的应用前景。
The formation mechanism of RSF alcogel was elucidated from morphological observations using TEM, AFM and confocal laser scanning microscopy (CLSM) as well as the kinetic studies by Thioflavin T fluorescence and rheological methods. An intriguing dual-structure—a nanofibrillar network on the nanoscale and a floc-like network on the microscale—was observed within the RSF alcogel. Based on our kinetic study, the fractal dimension of the ramified fibrillar aggregate was found to be 2.4–2.8 depending on RSF concentration. Meanwhile, through a scaling analysis of the mechanical properties of RSF alcogels, the fractal dimension of the floc-linked network was found to be 2.2. Furthermore, our results showed that fibrillarization of RSF was triggered by a dramatic change in the interaction between RSF and cosolvent, and the gelation kinetics could be tuned by adjusting the concentration of ethanol as well as RSF. The controllable dual-structure and kinetics make such biopolymer gel a potential candidate for applications in nanotechnology.