Exploring human plasma proteome strategies: High efficiency in-solution digestion protocol for multi-dimensional protein identification technology
Exploring human plasma proteome strategies: High efficiency in-solution digestion protocol for multi-dimensional protein identification technology
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DOI：
10.1016/j.chroma.2005.06.080
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发表时间：
2006-04-14
影响因子：
4.1
通讯作者：
Shriver, CD
中科院分区：
文献类型：
--
作者：
Ru, QHC;Zhu, LWA;Shriver, CD
多维蛋白质鉴定技术（MudPIT）以其高通量分离和精确质量检测的优势成为蛋白质组学研究的热点。在MudPIT分析之前，复杂的样品需要溶液中消化。与凝胶内消化不同，其中酶仅作用于少数蛋白质，溶液内消化涉及同时消化数百或数千种蛋白质。因此，溶液中消化方案必须非常有效。很少有研究评估了溶液中消化方案的效率。本研究比较了三个这样的协议。结果表明，利用三氟乙醇（TFE）作为变性剂的协议是最有效的。(c)2005 Elsevier B.V.保留所有权利。
Multi-dimensional protein identification technology (MudPIT) is becoming a prevalent proteomic approach due to its high-throughput separations and accurate mass detection. Prior to MudPIT analysis, complicated samples required in-solution digestion. Unlike in-gel digestion, in which enzymes work on just a few proteins, in-solution digestion involves simultaneous digestion of hundreds or thousands of proteins. In-solution digestion protocols must therefore be very efficient. Few investigations have evaluated the efficiency of in-solution digestion protocols. The present research compared three such protocols. Results suggest that a protocol utilizing trifluoroethanol (TFE) as denaturant is most efficient. (c) 2005 Elsevier B.V. All rights reserved.