The complete nucleotide sequence of a pathogenic swine vesicular disease virus isolated in Japan (J1'73) and phylogenetic analysis.
The complete nucleotide sequence of a pathogenic swine vesicular disease virus isolated in Japan (J1'73) and phylogenetic analysis.
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日本分离的致病性猪水泡病病毒（J173）的完整核苷酸序列和系统发育分析。
DOI：
10.1093/nar/21.16.3896
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发表时间：
1993
影响因子：
14.9
通讯作者：
T. Saeki
中科院分区：
文献类型：
--
作者：
T. Inoue;S. Yamaguchi;T. Kanno;S. Sugita;T. Saeki
我们克隆了在日本茨城县分离的致病性猪水泡病病毒 (SVDV) (Ji '73) (1) 的基因组 cDNA，并确定了完整的核苷酸序列。 cDNA文库由5个克隆组成，这些克隆相互重叠，覆盖了除5'末端区域65个碱基外的整个SVDV RNA序列。使用Sequenase试剂盒(美国生化公司，美国)在两个方向测定cDNA的序列，并通过直接RNA测序测定5'末端区域的序列。基因组长度为 7,401 个核苷酸，不包括 Poly(A) 尾。 DNA序列同源性为：与SVDV H3'76(2)同源性为98.9%，与SVDV UK'72(3)同源性为98.7%，与柯萨奇病毒B(CB)5(4)同源性为78.9%，与CB1(5)同源性为76.9%，与CB3(6)同源性为76.6%，与CB4(7)同源性为75.9%，与CB4(7)同源性为74.7%。柯萨奇病毒 A (CA) 9 (8) 和 61.9% 的脊髓灰质炎病毒 1 型 Mahoney (PVI) (9)。为了确定 SVDV 和其他相关肠道病毒之间的系统发育关系，我们使用邻接方法构建了进化树。图 1 表示其 1A (VP4) 序列的树。柯萨奇病毒之间的系统发育位置发生了变化
We have cloned the genomic cDNA of a pathogenic swine vesicular disease virus (SVDV) isolated in Ibaraki in Japan (Ji '73) (1) and the complete nucleotide sequence was determined. The cDNA library was composed of 5 clones, which were overlapped each other and covered the entire SVDV RNA sequence except 65 bases of 5' terminal region. The sequences of cDNA were determined in both directions using Sequenase kit (United States Biochemical Corporation, USA) and that of the 5' terminal region was determined by direct RNA sequencing. The genome is 7,401 nucleotides in length, excluding poly(A) tail. The DNA sequence homologies were 98.9% with SVDV H3'76 (2), 98.7% with SVDV UK'72 (3), 78.9% with coxsackievirus B (CB) 5 (4), 76.9% with CB1 (5), 76.6% with CB3 (6), 75.9% with CB4 (7), 74.7% with coxsackievirus A (CA) 9 (8) and 61.9% with poliovirus type 1 Mahoney (PVI) (9). To determine the phylogenetic relationships among SVDV and other related enteroviruses, we constructed evolutionary trees using neighbor-joining methods. Figure 1 represents the tree for their 1A (VP4) sequences. The phylogenetic positions among coxsackieviruses were changed