Cyclic AMP accumulation in psoriatic skin: differential responses to histamine, AMP, and einephrine by the uninvolved and involved epidermis.
Cyclic AMP accumulation in psoriatic skin: differential responses to histamine, AMP, and einephrine by the uninvolved and involved epidermis.
复制标题
银屑病皮肤中的环状 AMP 积累：未受累和受累表皮对组胺、AMP 和肾上腺素的不同反应。
DOI：
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发表时间：
1978
影响因子：
6.5
通讯作者：
V. Levine
中科院分区：
文献类型：
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作者：
H. Iizuka;K. Adachi;K. Halprin;V. Levine
利用银屑病患者的未受累和受累皮肤，我们研究了组胺和AMP（或腺苷）在体外对细胞内环AMP水平的影响。两种药物都激活了未参与和参与的腺苷酸环化酶，导致环AMP的积累。没有环核苷酸磷酸二酯酶（PDE）抑制剂，这些反应是双相的，在5分钟内观察到最大的积累。使用PDE抑制剂，这两种反应都显着增强，并且在超过20分钟的时间内观察到高水平的环AMP。受累皮肤对组胺的反应比未受累皮肤大得多。对腺苷的反应程度大致相等。根据我们之前的工作，受累皮肤对肾上腺素的反应比未受累皮肤要小得多。因此，银屑病患者受累皮肤的腺苷酸环化酶对肾上腺素的反应明显减弱，同时对组胺的反应明显增强。这排除了对肾上腺素无反应可能是由于表皮银屑病斑块细胞普遍无法形成功能细胞膜所致的可能性。
Using the uninvolved and involved skin from psoriatic patients, we investigated the effects of histamine and AMP (or adenosine) in vitro on the intracellular cyclic AMP levels. Both agents activated adenylate cyclase of the uninvolved and involved resulting in the accumulation of cyclic AMP. Without a cyclic nucleotide phosphodiesterase (PDE) inhibitor, these responses were biphasic and the maximal accumulation was observed in 5 min. With the PDE inhibitor both responses were markedly potentiated and high levels of cyclic AMP were observed for more than 20 min. The response to histamine by the involved skin was much greater than that by the uninvolved. The degree of the response to adenosine was approximately equal. In accordance with our previous work, the response to epinephrine by the involved skin was much less than that by the uninvolved. Thus adenylate cyclases of involved skin from psoriatic patients exhibit a markedly diminished response to epinephrine while at the same time exhibiting a markedly enhanced response to histamine. This precludes the possibility that the unresponsiveness to epinephrine can be due to a generalized inability of the epidermal psoriatic plaque cell to make a functioning cell membrane.