CYCLIC NUCLEOTIDE-GATED ION CHANNELs 14 and 16 Promote Tolerance to Heat and Chilling in Rice1[OPEN]
CYCLIC NUCLEOTIDE-GATED ION CHANNELs 14 and 16 Promote Tolerance to Heat and Chilling in Rice1[OPEN]
复制标题
环状核苷酸门控离子通道 14 和 16 提高水稻的耐热性和耐冷性(1)([OPEN])
DOI：
10.1104/pp.20.00591
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发表时间：
2020-08-01
期刊：
影响因子：
7.4
通讯作者：
Hua, Jian
中科院分区：
文献类型：
--
作者：
Cui, Yongmei;Lu, Shan;Hua, Jian
钙信号传导被认为对植物的耐热性和耐冷性至关重要，但其分子机制尚未完全清楚。本研究通过基因组编辑技术获得了两个CNGC蛋白OsCNGC 14和OsCNGC 16的功能缺失突变体，并对其在水稻耐温胁迫中的功能进行了研究。在高温和低温胁迫下，cngc14和cngc16突变体都表现出存活率降低，过氧化氢积累水平升高，细胞死亡增加。在ngc16突变体中，一些基因在热应激反应中被诱导和抑制的程度发生了改变，一些热休克因子（HSF）和热休克蛋白（HSP）基因的诱导程度略高于野生型。此外，OsCNGC 14或OsCNGC 16的缺失降低或消除了由热或冷胁迫诱导的胞浆钙信号。因此，OsCNGC 14和OsCNGC 16是植物耐热性和耐冷性所必需的，并且是响应温度胁迫的钙信号调节剂。此外，拟南芥中AtCNGC 2和AtCNGC 4同源物的缺失也导致了低温耐受性的降低。因此，本研究表明，CNGC基因在植物的耐冷性和耐热性中起着关键作用，表明在响应高温和低温胁迫的钙信号中可能存在重叠。
Calcium signaling has been postulated to be critical for both heat and chilling tolerance in plants, but its molecular mechanisms are not fully understood. Here, we investigated the function of two closely related cyclic nucleotide-gated ion channel (CNGC) proteins, OsCNGC14 and OsCNGC16, in temperature-stress tolerance in rice (Oryza sativa) by examining their loss-of-function mutants generated by genome editing. Under both heat and chilling stress, both thecngc14andcngc16mutants displayed reduced survival rates, higher accumulation levels of hydrogen peroxide, and increased cell death. In thecngc16mutant, the extent to which some genes were induced and repressed in response to heat stress was altered and someHeat Shock factor(HSF) andHeat Shock Protein(HSP) genes were slightly more induced compared to the wild type. Furthermore, the loss of eitherOsCNGC14orOsCNGC16reduced or abolished cytosolic calcium signals induced by either heat or chilling stress. Therefore,OsCNGC14andOsCNGC16are required for heat and chilling tolerance and are modulators of calcium signals in response to temperature stress. In addition, loss of their homologsAtCNGC2andAtCNGC4in Arabidopsis (Arabidopsis thaliana) also led to compromised tolerance of low temperature. Thus, this study indicates a critical role ofCNGCgenes in both chilling and heat tolerance in plants, suggesting a potential overlap in calcium signaling in response to high- and low-temperature stress.Cyclic nucleotide-gated ion channels in rice promote tolerance to both heat and chilling and affect the calcium response to temperature stresses.