Effects of suppression of chloroplast phosphoglycerate kinase on photosynthesis in rice
Effects of suppression of chloroplast phosphoglycerate kinase on photosynthesis in rice
复制标题
抑制叶绿体磷酸甘油酸激酶对水稻光合作用的影响
DOI：
10.1007/s11120-022-00923-w
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发表时间：
2022
影响因子：
3.7
通讯作者：
Makino A
中科院分区：
文献类型：
--
作者：
Suzuki Y;Konno Y;Takegahara-Tamakawa Y;Miyake C;Makino A
由于叶绿体磷酸甘油酸激酶（cpPGK）是Calvin-Benson循环酶中容量最高的酶之一，它尚未被认为是光合能力的决定因素。然而，据报道，在高光照和正常[CO2]水平下，拟南芥ppgk基因下调突变体的CO2同化速率下降，这表明在正常[CO2]水平下，cpPGK对光合能力有控制作用。本研究在高光照和不同[CO2]水平下，研究了cpPGK蛋白含量降低的转基因水稻植株cpPGK对光合能力的贡献。使用RNA干扰技术抑制了编码cppgk的基因。获得了2个转基因株系Pi3和Pi5，其cpPGK蛋白含量分别下降到野生型的21%和76%。然而，野生型和转基因植株在co2同化速率上没有显著差异。在[CO2]浓度升高时，转基因系Pi3的CO2同化率仅略有下降，而在野生型植物和转基因系Pi5之间没有差异，与[CO2]浓度无关。这些结果清楚地表明，在不同[CO2]水平下，cpPGK对水稻光合能力没有很强的控制作用。
As chloroplast phosphoglycerate kinase (cpPGK) is one of the enzymes which has the highest capacity among the Calvin–Benson cycle enzymes, it has not been regarded as a determinant for photosynthetic capacity. However, it was reported that the rate of CO2assimilation decreased under high irradiance and normal [CO2] levels in the ArabidopsiscpPGK-knockdown mutant, implying that cpPGK has a control over photosynthetic capacity at a normal [CO2] level. In the present study, the contribution of cpPGK to photosynthetic capacity was evaluated in transgenic rice plants with decreased amounts of cpPGK protein under high irradiance and various [CO2] levels. The gene encodingcpPGKwas suppressed using RNA interference techniques. Two lines of transgenic plants, Pi3 and Pi5, in which the amount of cpPGK protein decreased to 21% and 76% of that in wild-type plants, respectively, were obtained. However, there was no substantial difference in the rates of CO2assimilation between wild-type and transgenic plants. The rates of CO2assimilation decreased only slightly at elevated [CO2] levels in the transgenic line Pi3 and did not differ between wild-type plants and the transgenic line Pi5, irrespective of [CO2] level. These results clearly indicate that cpPGK does not have a strong control over photosynthetic capacity at various [CO2] levels in rice.
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影响因子：
4.3
作者：
M. Paul;S. Driscoll;P. J. Andralojc;J. S. Knight;J. Gray;D. Lawlor
通讯作者：
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DOI：
10.1071/pp9950285
发表时间：
1995
期刊：
Australian Journal of Plant Physiology
影响因子：
--
作者：
G. Price;Jian;S. Caemmerer;J. R. Evans;W. Chow;Jan M. Anderson;V. Hurry;Badger
通讯作者：
Badger
叶绿体中 3-磷酸甘油酸还原的酶促控制
DOI：
10.1016/0005-2728(92)90071-9
发表时间：
1992
期刊：
Biochimica et Biophysica Acta
影响因子：
--
作者：
L. Fridlyand
通讯作者：
L. Fridlyand
影响因子：
4.3
作者：
EVANS, JR
通讯作者：
EVANS, JR
DOI：
10.1071/pp97164
发表时间：
1998-01-01
期刊：
AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY
影响因子：
--
作者：
Price, GD;von Caemmerer, S;Badger, MR
通讯作者：
Badger, MR