Assessment of soil microbial diversity measurements as indicators of soil functioning in organic and conventional horticulture systems
Assessment of soil microbial diversity measurements as indicators of soil functioning in organic and conventional horticulture systems
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DOI：
10.1016/j.ecolind.2011.02.008
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发表时间：
2011-09-01
影响因子：
6.9
通讯作者：
Nannipieri, Paolo
中科院分区：
文献类型：
--
作者：
Romaniuk, Romina;Giuffre, Lidia;Nannipieri, Paolo
土壤质量评价是检测不同管理措施下土壤性质变化的必要手段。一些微生物特性可能有助于预测土壤的变化，从而为土壤功能提供一个综合和相关的视角。这项工作的目的是评估和比较不同的方法来评估微生物多样性，如甲基酯脂肪酸（PLEA）和分解代谢反应谱（CRP）作为土壤功能的指标。本研究是在集约化园艺生产系统中进行的。研究了未扰动土壤、5年试验小区和20年以上试验小区在有机生产和常规生产条件下的变化。主成分分析，然后多元判别分析表明，D-葡萄糖，D-氨基葡萄糖，α-酮丁酸，α-酮戊二酸和尿酸的底物具有最高的敏感性，以单独的情况。对PLEA进行了相同的分析，表明C18：1 w 9、C13：0、C16：1 w 9、C14：0、i15：0和cy 19：0甲酯脂肪酸最敏感。所选底物和脂肪酸的多变量方差分析表明，CRP和PLEA技术都能够表征所研究的系统。常规管理小区的饱和/单不饱和（S/M）、异/反异（i/a）和环丙基/前体（cy/pre）微生物胁迫指标较高，也表现出这些情况下微生物生物量和真菌/细菌比（F/B）最低，特别是在常规管理20年以上的小区。微生物功能多样性，计算均匀度（E）从CRP是能够区分所有的情况和管理系统，显示这种测量作为土壤功能的综合指标的潜力。(C)2011爱思唯尔有限公司保留所有权利。
Soil quality assessment is necessary to detect changes on soil properties among different management practices. Some microbial properties could be useful to predict changes in soil providing an integrated and relevant vision of soil functioning. The aim of this work was to evaluate and compare different methods to assess microbial diversity, such as methyl ester fatty acids (PLEA) and catabolic response profiles (CRP) to act as indicators of soil functioning. The study was carried out in an intensive horticulture production system. Undisturbed soil, 5 years plots, and more than 20 years plots under organic and conventional production were studied. Principal component analysis followed by multivariate discriminate analysis showed that D-glucose, D-glucosamine, alpha-ketobutyric, alpha-ketoglutaric and uric acids were the substrates with the highest sensitivity to separate situations. The same analysis was performed for PLEA, showing that C18:1w9, C13:0, C16:1w9, C14:0, i15:0 and cy19:0 methyl ester fatty acids were the most sensitive. Multivariate analysis of variance of selected substrates and fatty acids showed that CRP and PLEA techniques were both capable to characterize the studied systems. Saturated/monounsaturated (S/M), iso/anteiso (i/a) and cyclopropyl/precursors (cy/pre) microbial stress indicators were higher in plots under conventional management, presenting also these situations the lowest microbial biomass and fungi/bacteria ratio (F/B), especially in plots under conventional management for more than 20 years. Microbial functional diversity, calculated as evenness (E) from CRP was capable to distinguish between all situations and management systems showing the potential of this measurement to act as an integrative indicator of soil functioning. (C) 2011 Elsevier Ltd. All rights reserved.