Asiaticoside Mitigates the Allergic Inflammation by Abrogating the Degranulation of Mast Cells
Asiaticoside Mitigates the Allergic Inflammation by Abrogating the Degranulation of Mast Cells
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积雪草苷通过消除肥大细胞脱粒来减轻过敏性炎症。
DOI：
10.1021/acs.jafc.7b01590
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发表时间：
2017-09-20
影响因子：
6.1
通讯作者：
Yan, Guang Hai
中科院分区：
文献类型：
--
作者：
Jiang, Jing Zhi;Ye, Jing;Yan, Guang Hai
研究了积雪苷（AS）对肥大细胞介导的变态反应的影响。AS对大鼠腹腔肥大细胞无明显细胞毒性作用。AS可降低RPMC细胞内钙离子浓度，抑制组胺释放和脱颗粒。AS还降低了IgE致敏的RBL-2 H3细胞中抗原诱导的肿瘤坏死因子α、白细胞介素（IL）-4、IL-8和IL-1 β的产生。AS对促炎细胞因子的抑制作用与激活细胞内FceRI和抑制核因子-κ B信号通路有关。此外，AS使抗原诱导的Syk、林恩、Gab 2和PLC γ 1的磷酸化失效，从而抑制下游Akt磷酸化和MAPK途径。HO-1和Nrf 2表达呈时间依赖性增加。综上所述，我们证明AS抑制肥大细胞介导的过敏性炎症，这种作用可能是通过Fc ε RI依赖的信号通路介导的。
The effects of asiaticoside (AS) on allergic responses mediated by mast cells were investigated. AS showed no obvious cytotoxicity on RPMCs (rat peritoneal mast cells). AS reduced the intracellular calcium in RPMCs and deprived the histamine release and degranulation. AS also decreased the generation of antigen-induced tumor necrosis factor alpha, interleukin (IL)-4, IL-8, and IL-1 beta in RBL-2H3 cells sensitized by IgE. The suppression of AS on pro-inflammatory cytokines was related with the activation of the intracellular FceRI and the inhibition of the nuclear factor-kappa B signaling pathway. In addition, AS disabled the phosphorylation of antigen-induced Syk, Lyn, Gab2, and PLC gamma 1, thus suppressing the downstream Akt phosphorylation and MAPKs pathways. It also increased HO-1 and Nrf2 expression time dependently. In summary, we demonstrate that AS suppresses the allergic inflammation mediated by mast cells and this effect might be mediated by Fc epsilon RI-dependent signaling pathways.