Diphtheria toxin: mode of action and structure
Diphtheria toxin: mode of action and structure
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DOI：
10.1128/br.39.1.54-85.1975
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发表时间：
1975-03
影响因子：
--
通讯作者：
R. Collier
中科院分区：
文献类型：
--
作者：
R. Collier
引言.... 54 问题概述。 54 蛋白质合成的抑制..56 细胞培养研究..56 无细胞系统研究。 8 EF-2 的 ADP-核糖基化 .59 反应的鉴定。 ADPR 是共价连接的。 .60 毒素的作用是催化作用。 1 底物特异性 ..6 可逆性和热力学 .62 影响正向反应速率的因素 62 毒素的结构-活性关系 63 整个毒素是一种酶原 ..63 各种片段和形式的毒性。 65 片段 A 的特性 ..65 毒素制剂的变化 .66 无毒、交叉反应的毒素形式。 7 ADP-核糖基化反应的机制 ..68 整个毒素的吡啶核苷酸结合和 ADP-核糖基化活性 ... 70 ADP-核糖基化反应是否会产生毒性？ ... 71 细胞中 ADP-核糖化反应的事件.. 72 与特定受体的附着.72 进入和激活过程 3 进入和周转的动力学 4 免疫学.75 亲合力与针对片段 B.75 的抗体的免疫原性和类毒素机制相关。白喉毒素的生物学功能和来源.77 生物学功能.7 来源... 7 总结和结论性意见....................................... 78 引用的文献.................................................... 80
INTRODUCTION.... 54 OUTLINE OF THEPROBLEM. 54 INHIBITION OFPROTEIN SYNTHESIS ..56 Studies in Cell Culture ..56 Studies in Cell-Free Systems . 8 ADP-RIBOSYLATION OF EF-2 .59 Identification of the Reaction . ADPR is Covalently Attached . .60 The Role of Toxin is Catalytic . 1 Substrate Specificity ..6 Reversibility and Thermodynamics .62 Factors Affecting the Rate of the Forward Reaction 62 STRUCTURE-ACTIVITY RELATIONSHIPS IN THE TOXIN 63 Whole Toxin is a Proenzyme ..63 Toxicity of the Various Fragments andForms. 65 Properties of Fragment A ..65 Variations Among Toxin Preparations .66 Nontoxic, Cross-Reacting Forms of Toxin . 7 Mechanism of the ADP-Ribosylation Reaction ..68 Pyridine Nucleotide Binding and ADP-Ribosylation Activity of Whole Toxin ... 70 IS THE ADP-RIBOSYLATION REACTION RESPONSIBLE FOR TOXICITY? ... 71 EVENTSPRECEDING THE ADP-RIBOSYLATION REACTION IN CELLS.. 72 Attachment to Specific Receptors.72 Entry and Activation Processes 3 Kinetics of Entry and Turnover 4 IMMUNOLOGY.75 Avidity Correlates with Antibody Against Fragment B.75 Immunogenicity and the Mechanism of Toxoiding. BIOLOGICAL FUNCTION AND ORIGIN OF DIPHTHERIATOXIN.77 Biological Function.7 Origin... 7 SUMMARY AND CONCLUDING REMARKS.................................. 78 LITERATURE CITED............................... 80