EFFECTS OF SPACEFLIGHT ON THE NUMBER OF RAT PERIPHERAL-BLOOD LEUKOCYTES AND LYMPHOCYTE SUBSETS
EFFECTS OF SPACEFLIGHT ON THE NUMBER OF RAT PERIPHERAL-BLOOD LEUKOCYTES AND LYMPHOCYTE SUBSETS
复制标题
DOI：
10.1002/jlb.55.2.209
复制
发表时间：
1994-02-01
影响因子：
5.5
通讯作者：
LANGE, RD
中科院分区：
文献类型：
--
作者：
ALLEBBAN, Z;ICHIKI, AT;LANGE, RD
用29只雄性大鼠的外周血白细胞和脾淋巴细胞进行实验，这些大鼠是1991年6月在哥伦比亚号航天飞机上执行为期9天的空间实验室生命科学1号任务期间飞行的，并在适当的地面对照上进行了实验。在着陆当天，飞行动物的白细胞总数(P<；0.0001)与对照组相比显著下降。飞行动物的淋巴细胞绝对数(P<；0.0001)和单核细胞绝对数(P<；0.0001)也显著降低。与飞行前相比，降落时嗜酸性粒细胞的绝对数量略有减少，中性粒细胞的数量略有增加。对飞行动物和对照动物外周血和脾淋巴细胞的免疫表型分析表明，在着陆当天，CD_4、CD_8阳性细胞和B淋巴细胞的绝对数减少。然而，外周血中CD4+、CD8+和B细胞的相对百分比并未被发现受到抑制。飞行动物的脾淋巴细胞反应性百分率与对照组相比无明显差异。在飞行后即刻观察到的白细胞和淋巴细胞数量的下降是一过性的，所有数值在飞行后9天恢复到对照水平。
Experiments were carried out on peripheral blood leukocytes and spleen lymphocytes from 29 male rats that were flown during the Spacelab Life Sciences 1 (SLS-1) nine-day mission on the shuttle Columbia in June 1991 and on appropriate ground controls. On the day of landing, there was a significant decrease in the total white blood cell counts (P < 0.0001) of flight animals in comparison to controls. There was also a significant decrease in the absolute number of lymphocytes (P < 0.0001) and monocytes (P < 0.0001) in the flight animals. A slight decrease in the absolute number of eosinophils and a slight increase in the number of neutrophils were observed at landing, compared with preflight values. Immunophenotyping of the peripheral blood and spleen lymphocytes of flight and control animals indicated that, on the day of landing, there was a decrease in the absolute number of CD4 and CD8 positive cells and B lymphocytes. However, relative percentages of peripheral blood CD4+, CD8+, and B cells were not found to be depressed. No differences were discerned in the percent reactivity of spleen lymphocytes of flight animals compared with controls. The observed decrease in the number of leukocytes and lymphocytes at the immediate postflight period was transient and all values returned to the control levels by nine days postflight.