Capsule-transmitted gut symbiotic bacterium of the Japanese common plataspid stinkbug, Megacopta punctatissima
Capsule-transmitted gut symbiotic bacterium of the Japanese common plataspid stinkbug, Megacopta punctatissima
复制标题
DOI：
10.1128/aem.68.1.389-396.2002
复制
发表时间：
2002-01-01
影响因子：
4.4
通讯作者：
Hosokawa, T
中科院分区：
文献类型：
--
作者：
Fukatsu, T;Hosokawa, T
日本常见的平盾蝽Megacopta punctatissima将棕色小颗粒或共生体胶囊沉积在卵团的底部，目的是将共生细菌传播给后代。我们调查了微生物学方面的胶囊中所含的细菌，如微生物多样性，系统发育的位置，在体内定位，和健身对宿主昆虫的影响。16S核糖体DNA克隆的限制性片段长度多态性分析显示，一个单一的细菌物种占主导地位的微生物群中的胶囊。在卵中没有检测到这种细菌，但在胶囊中，这明确表明，通过探测胶囊内容物，细菌是经口而不是经卵传播给昆虫后代的。分子系统发育分析表明，该细菌属于变形菌门的γ亚门。在成年昆虫中，细菌位于中肠的后部。从该虫体上去除该细菌后，可导致发育迟缓、生长停滞、体色异常等症状，表明该细菌对宿主昆虫的正常发育和生长至关重要。
The Japanese common plataspid stinkbug, Megacopta punctatissima, deposits small brown particles, or symbiont capsules, on the underside of the egg mass for the purpose of transmission of symbiotic bacteria to the offspring. We investigated the microbiological aspects of the bacteria contained in the capsule, such as microbial diversity, phylogenetic placement, localization in vivo, and fitness effects on the host insect. Restriction fragment length polymorphism analysis of 16S ribosomal DNA clones revealed that a single bacterial species dominates the microbiota in the capsule. The bacterium was not detected in the eggs but in the capsules, which unequivocally demonstrated that the bacterium is transmitted to the offspring of the insect orally rather than transovarially, through probing of the capsule content. Molecular phylogenetic analysis showed that the bacterium belongs to the gamma-subdivision of the Proteobacteria. In adult insects the bacterium was localized in the posterior section of the midgut. Deprivation of the bacterium from the nymphs resulted in retarded development, arrested growth, abnormal body coloration, and other symptoms, suggesting that the bacterium is essential for normal development and growth of the host insect.