Estrogen modulation of human breast cancer cell growth
Estrogen modulation of human breast cancer cell growth
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DOI：
10.1007/bf02975213
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发表时间：
1997-12-01
影响因子：
6.7
通讯作者：
Sheen, YY
中科院分区：
文献类型：
--
作者：
Lee, HO;Sheen, YY
为了进一步了解雌激素如何调节细胞功能，在人乳腺癌细胞中研究了雌激素对细胞增殖的影响。我们研究了雌激素对三种雌激素受体含量不同的人乳腺癌细胞系增殖的影响。10 nM雌二醇显著刺激MCF-7人乳腺癌细胞的增殖，MCF-7人乳腺癌细胞含有高水平的雌激素受体（1.15+/-0.03 pmole/mg蛋白质）。在含有低水平雌激素受体的T47 D细胞中（0. 23+/-0.05 pmole/mg蛋白质），10 nM雌激素与对照相比轻微刺激增殖。MDA-MB-231细胞不含可检测水平的雌激素受体，其生长不受雌激素的影响。这些结果表明，它们对雌激素刺激生长的敏感性与它们的雌激素受体含量密切相关。我们还研究了雌激素对MCF-7细胞孕酮受体水平和纤溶酶原激活物活性的影响。10 nM雌二醇对MCF-7细胞中孕酮受体水平和纤溶酶原激活物活性的刺激最大。雌激素对孕酮受体和纤溶酶原激活物活性的刺激是否与雌激素刺激MCF-7细胞增殖有关尚不清楚。对培养的人乳腺癌细胞中雌激素的研究表明，对雌激素刺激生长的敏感性与雌激素受体含量密切相关。
To gain further insight into how estrogens modulate cell function, the effects of estrogen on cell proliferation were studied in human breast cancer cells. We examined the effects of estrogen on the proliferation of three human breast cancer cell lines that differed in their estrogen receptor contents. Ten nM estradiol markedly stimulated the proliferation of MCF-7 human breast cancer cells that contained high levels of estrogen receptor (1.15+/-0.03 pmole/mg protein) over that of control. In T47D cells that contained low levels of estrogen receptor (0. 23+/-0.05 pmole/mg protein), Ten nM estrogen slightly stimulated the proliferation over that of control. MDA-MB-231 cells, that contained no detectable levels of estrogen receptors, had their growth unaffected by estrogen. These results showed their sensitivity to growth stimulation by estrogen correlated well with their estrogen receptor content. Also we examined the effect of estrogen on cellular progesterone receptor level as well as plasminogen activator activity in MCF-7 cells. Ten nM estradiol showed maximal stimulation of progesterone receptor level as well as plasminogen activator activity in MCF-7 cells. It is not clear whether these stimulations of progesterone receptor and plasminogen activator activity by estrogen are related to the estrogen stimulation of cell proliferation of MCF-7 cells. Studies with estrogen in human breast cancer cells in culture indicate that sensitivity to growth stimulation by estrogen correlates well with estrogen receptor contents.