A YEAST CHROMOSOMAL ORIGIN OF DNA-REPLICATION DEFINED BY MULTIPLE FUNCTIONAL ELEMENTS
A YEAST CHROMOSOMAL ORIGIN OF DNA-REPLICATION DEFINED BY MULTIPLE FUNCTIONAL ELEMENTS
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DOI：
10.1126/science.1536007
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发表时间：
1992-02-14
期刊：
影响因子：
56.9
通讯作者：
STILLMAN, B
中科院分区：
文献类型：
--
作者：
MARAHRENS, Y;STILLMAN, B
虽然已经证明，DNA复制的离散起点存在于真核细胞染色体中，但真核细胞起源的详细结构仍有待确定。在整个酿酒酵母染色体起源ARS 1中构建接头取代突变。对这些突变体的功能研究揭示了一个基本元件（A），其包括与ARS共有序列的匹配，以及三个额外的元件（B1、B2和B3），它们共同对起源功能也是必需的。这四个元件的排列与ARS 1完全相同，但被完全不相关的序列包围，作为一个有效的起源。元件B3是转录因子源结合蛋白ABF1的结合位点。其他转录因子结合位点取代B3元件，并且需要反式作用转录激活结构域。染色体复制起点的多体性质和转录激活因子在其功能中的作用与真核启动子的组织有惊人的相似性。
Although it has been demonstrated that discrete origins of DNA replication exist in eukaryotic cellular chromosomes, the detailed organization of a eukaryotic cellular origin remains to bc determined. Linker substitution mutations were constructed across the entire Saccharomyces cerevisiae chromosomal origin, ARS1. Functional studies of these mutants revealed one essential clement (A), which includes a match to the ARS consensus sequence, and three additional elements (B1, B2, and B3), which collectively are also essential for origin function. These four elements arranged exactly as in ARS1, but surrounded by completely unrelated sequence, functioned as an efficient origin. Element B3 is the binding site for the transcription factor-origin binding protein ABF1. Other transcription factor binding sites substitute for the B3 element and a trans-acting transcriptional activation domain is required. The multipartite nature of a chromosomal replication origin and the role of transcriptional activators in its function present a striking similarity to the organization of eukaryotic promoters.