Comprehensive analysis of immune infiltration and gene expression for predicting survival in patients with sarcomas.
Comprehensive analysis of immune infiltration and gene expression for predicting survival in patients with sarcomas.
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综合分析免疫浸润和基因表达来预测肉瘤患者的生存
DOI：
10.18632/aging.202229
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发表时间：
2020-12-09
期刊：
影响因子：
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通讯作者：
Wang J
中科院分区：
文献类型：
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作者：
Chen H;Song Y;Deng C;Xu Y;Xu H;Zhu X;Song G;Tang Q;Lu J;Wang J
肿瘤微环境与肿瘤发生密切相关，免疫浸润细胞和免疫相关分子是潜在的预后标志物。然而，传统测量方法的缺点限制了对肿瘤微环境中各种成分的准确评价。随着新一代RNA测序技术的快速发展，已经实现了对肿瘤微环境中免疫过滤的专门和深入分析。在这项研究中，我们结合生物信息学分析方法ESTIMATE，CIBERSORT和ssGSEA来表征肉瘤的免疫浸润，并确定不同病理亚型的特异性免疫调节剂。我们进一步提取了与改善预后相关的重要免疫相关基因的功能富集，包括NR1H3，VAMP5，GIMAP2，GBP2，HLA-E和CRIP1。总的来说，免疫微环境是肉瘤的重要预后决定因素，可能是开发有效免疫疗法的潜在资源。
Tumor microenvironments are strongly related to tumor development, and immune-infiltrating cells and immune-related molecules are potential prognostic markers. However, the shortcomings of traditional measurement methods limit the accurate evaluation of various components in tumor microenvironments. With the rapid advancement of Next-Generation RNA Sequencing technology, dedicated and in-depth analyses of immune filtration within the tumor microenvironment has been achieved. In this study, we combined the bioinformatics analysis methods ESTIMATE, CIBERSORT, and ssGSEA to characterize the immune infiltration of sarcomas and to identify specific immunomodulators of different pathological subtypes. We further extracted a functional enrichment of significant immune-related genes related to improved prognosis, including NR1H3, VAMP5, GIMAP2, GBP2, HLA-E and CRIP1. Overall, the immune microenvironment is an important prognostic determinant of sarcomas and may be a potential resource for developing effective immunotherapy.