Morphological Characters and Transcriptome Profiles Associated with Black Skin and Red Skin in Crimson Snapper (Lutjanus erythropterus).
Morphological Characters and Transcriptome Profiles Associated with Black Skin and Red Skin in Crimson Snapper (Lutjanus erythropterus).
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与红鲷鱼（Lutjanus erythropterus）黑皮和红皮相关的形态特征和转录组谱
DOI：
10.3390/ijms161126005
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发表时间：
2015-11-12
影响因子：
5.6
通讯作者：
Liu CW
中科院分区：
文献类型：
--
作者：
Zhang YP;Wang ZD;Guo YS;Liu L;Yu J;Zhang S;Liu SJ;Liu CW
本研究对红鳍笛卡尔(Lutjanus Red Pterus)黑色区和红区两种不同颜色的皮肤进行了形态观察和光照测序。皮肤中有三种类型的生色团，即黑素团、虹膜生色团和黄发生团。两种颜色的主要区别在于三种发色团的数量和分布。比较两种转录本后，发现9200个表达差异显著(比值变化≥2和Q值≤0.05)的基因，其中5 972个在黑色皮肤上调，3 2 2 8个在红色皮肤上调。通过对差异转录基因的功能注释、基因本体论(GO)和京都基因与基因组百科全书(KEGG)途径分析，我们挖掘了一些未鉴定的候选色素基因，并发现了影响深红鱼色素形成的保守基因。通过对两种颜色的皮肤进行实时定量聚合酶链式反应分析，确定了14个色素基因的表达模式。总体而言，本研究对深红笛卡尔不同颜色皮肤的形态特征和转录组分析进行了全球调查，为揭示笛卡尔色素模式的形成机制提供了有价值的细胞和遗传信息。
In this study, morphology observation and illumina sequencing were performed on two different coloration skins of crimson snapper (Lutjanus erythropterus), the black zone and the red zone. Three types of chromatophores, melanophores, iridophores and xanthophores, were organized in the skins. The main differences between the two colorations were in the amount and distribution of the three chromatophores. After comparing the two transcriptomes, 9200 unigenes with significantly different expressions (ratio change ≥ 2 and q-value ≤ 0.05) were found, of which 5972 were up-regulated in black skin and 3228 were up-regulated in red skin. Through the function annotation, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of the differentially transcribed genes, we excavated a number of uncharacterized candidate pigment genes as well as found the conserved genes affecting pigmentation in crimson snapper. The patterns of expression of 14 pigment genes were confirmed by the Quantitative real-time PCR analysis between the two color skins. Overall, this study shows a global survey of the morphological characters and transcriptome analysis of the different coloration skins in crimson snapper, and provides valuable cellular and genetic information to uncover the mechanism of the formation of pigment patterns in snappers.