The effects of long-term administration of tadalafil on STZ-induced diabetic rats with erectile dysfunction via a local antioxidative mechanism
The effects of long-term administration of tadalafil on STZ-induced diabetic rats with erectile dysfunction via a local antioxidative mechanism
复制标题
长期服用他达拉非通过局部抗氧化机制对STZ诱导的糖尿病大鼠勃起功能障碍的影响
DOI：
10.1038/aja.2012.22
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发表时间：
2012-07-01
影响因子：
2.9
通讯作者：
Wang, Run
中科院分区：
文献类型：
--
作者：
Chen, Yun;Li, Xiao-Xin;Wang, Run
众所周知，5型磷酸二酯酶抑制剂（PDE 5 I）通过一氧化氮和环磷酸鸟苷（NO-cGMP）途径有效，并广泛用于治疗糖尿病勃起功能障碍（艾德）。然而，目前尚不清楚其他途径是否可能参与PDE 5 I治疗糖尿病艾德。本研究的目的是通过长期给予PDE 5 I，阐明抗氧化剂在糖尿病艾德治疗中的作用。采用三组Sprague-Dawley大鼠：N组，正常对照; D组，链脲佐菌素（STZ）诱导的糖尿病大鼠作为对照; D+T组，STZ诱导的糖尿病大鼠接受口服他达拉非8周。在安乐死前电刺激海绵体神经期间，通过海绵体内压（ICP）和平均动脉压（MAP）评估勃起功能。通过生化分析测定海绵体组织中丙二醛（MDA）、超氧化物歧化酶（SOD）和线粒体膜电位（MMP）的水平。电镜观察线粒体形态。ICP/MAP比值D+T组高于D组（P
Type 5 phosphodiesterase inhibitors (PDE5Is) are well known being effective via the nitric oxide and cyclic guanosine monophosphate (NO-cGMP) pathway and are widely used in the treatment of diabetic erectile dysfunction (ED). However, it is unclear whether other pathways may be involved in the treatment of diabetic ED with PDE5Is. The purpose of this study was to clarify the role of antioxidants in diabetic ED treatment through the long-term administration of PDE5Is. Three groups of Sprague-Dawley rats were utilized: Group N, the normal control; Group D, streptozotocin (STZ)-induced diabetic rats as a control; and Group D+T, STZ-induced diabetic rats who received oral administration of tadalafil for 8 weeks. Erectile function was assessed by intracavernous pressure (ICP) and mean arterial pressure (MAP) during electrical stimulation of the cavernous nerve before euthanasia. The levels of malondialdehyde (MDA), superoxide dismutase (SOD) and mitochondrial membrane potential (MMP) of cavernous tissue were assessed by biochemical analysis. The morphology of mitochondria was observed by electron microscopy. The ICP/MAP ratio was higher in Group D+T than in Group D (P