Cross amplification of microsatellite loci developed for Atractosteus spatula in Atractosteus tropicus

Cross amplification of microsatellite loci developed for Atractosteus spatula in Atractosteus tropicus
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为 Atractosteus tropicus 中的 Atractosteus spatula 开发的微卫星位点的交叉扩增

DOI:
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发表时间:
2013
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影响因子:
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通讯作者:
B. Kreiser
B. Kreiser
中科院分区:
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文献类型:
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作者:
Sandra Bohn;E. Barraza;Caleb D. McMahan;Wilfredo A. Matamoros;B. Kreiser

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由于近年来热带雀鳝(学名:Atractosteus tropicus)的种群数量下降,需要对其种群结构有更深入的了解。获得这种理解的一个关键步骤是微卫星位点的发展,用于这一物种。为此,利用33个微卫星位点对扬子鳄(A.抹刀)在来自萨尔瓦多的Zanjon del奇诺种群的52个个体中进行筛选。其中25个位点在该物种中成功扩增,其中9个位点在该群体中具有多态性。这些位点将为A基因分型提供有用的工具。在研究现有野生种群和监测水产养殖的遗传多样性方面,
Due to recent population declines in tropical gar (Atractosteus tropicus), a greater understanding of its population structure is needed. A key step in gaining this understanding is the development of microsatellite loci for use in this species. For this purpose, 33 microsatellite loci from alligator gar (A. spatula) were screened in 52 individuals from a population in Zanjon del Chino, El Salvador. Twenty-five of these loci successfully amplified in this species, and 9 of those loci were polymorphic in this population. These loci should provide a useful tool for genotyping A. tropicus, both in studying existing wild populations and in monitoring genetic diversity in aquaculture.