Assessing the Applicability of FISH-based Prematurely Condensed Dicentric Chromosome Assay in Triage Biodosimetry
Assessing the Applicability of FISH-based Prematurely Condensed Dicentric Chromosome Assay in Triage Biodosimetry
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DOI：
10.1097/hp.0000000000000182
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发表时间：
2015-03
期刊：
影响因子：
2.2
通讯作者：
Y. Suto;Takaya Gotoh;Takashi Noda;Miho Akiyama;M. Owaki;F. Darroudi;M. Hirai
中科院分区：
文献类型：
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作者：
Y. Suto;Takaya Gotoh;Takashi Noda;Miho Akiyama;M. Owaki;F. Darroudi;M. Hirai
双着丝粒染色体分析（DCA）被认为是辐射生物剂量学的金标准。然而，该试验需要在开始中期染色体分析之前进行2天外周血淋巴细胞培养，以估计生物剂量。其他生物测定也具有关于获得剂量估计所需的时间的缺点，所述剂量估计用于在正确的医学治疗线上快速决定。因此，需要适合分诊生物剂量测定要求的替代技术。辐射诱导的G 0期淋巴细胞DNA双链断裂可通过细胞融合介导的过早染色体凝集（PCC）方法检测为间期染色体畸变。该方法，结合荧光原位杂交（FISH）技术，已提出在早期的研究中作为一个强大的工具，获得生物剂量估计没有2-D淋巴细胞培养程序。目前的工作评估的适用性FISH为基础的PCC技术，使用泛着丝粒和端粒肽核酸（PNA）探针分诊模式的生物剂量测定，并表明，一个改进的快速程序的过早浓缩双着丝粒染色体（PCDC）测定有可能在短至6小时后，外周血标本的收集暴露的辐射剂量进行评估。
AbstractThe dicentric chromosome assay (DCA) has been regarded as the gold standard of radiation biodosimetry. The assay, however, requires a 2-d peripheral blood lymphocyte culture before starting metaphase chromosome analyses to estimate biological doses. Other biological assays also have drawbacks with respect to the time needed to obtain dose estimates for rapid decision on the correct line of medical treatment. Therefore, alternative technologies that suit requirements for triage biodosimetry are needed. Radiation-induced DNA double strand breaks in G0 lymphocytes can be detected as interphase chromosome aberrations by the cell fusion-mediated premature chromosome condensation (PCC) method. The method, in combination with fluorescence in situ hybridization (FISH) techniques, has been proposed in early studies as a powerful tool for obtaining biological dose estimates without 2-d lymphocyte culture procedures. The present work assesses the applicability of FISH-based PCC techniques using pan-centromeric and telomeric peptide nucleic acid (PNA) probes in triage mode biodosimetry and demonstrates that an improved rapid procedure of the prematurely condensed dicentric chromosome (PCDC) assay has the potential for evaluating exposed radiation doses in as short as 6 h after the collection of peripheral blood specimens.