Ecdysteroid promotes cell cycle progression in the Bombyx wing disc through activation of c-Myc
Ecdysteroid promotes cell cycle progression in the Bombyx wing disc through activation of c-Myc
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DOI：
10.1016/j.ibmb.2015.11.008
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发表时间：
2016-03-01
影响因子：
3.8
通讯作者：
Kawasaki, Hideki
中科院分区：
文献类型：
--
作者：
Moriyama, Minoru;Osanai, Kohji;Kawasaki, Hideki
成虫原基由生长向变态的发育转换是全变态昆虫成虫身体规划的一个重要过程。虽然它是纪律的蜕皮激素的顺序行动，分子机制连接到细胞增殖知之甚少。以家蚕末龄幼虫翅盘为材料，研究蜕皮激素对细胞周期相关基因表达的调控。我们发现，c-myc的表达水平显着升高，在饲养后细胞增殖阶段，这与蜕皮激素滴度的小幅增加相吻合。体外翅盘培养显示，补充中等水平的蜕皮激素在1小时内上调c-myc表达，随后增加细胞周期核心调节因子的表达，包括A-、B-、D-和E-型细胞周期蛋白基因Cdc 25和E2 F1。我们证明，c-myc上调的蜕皮甾体的蛋白质合成抑制剂的存在下，不受抑制，这表明蜕皮甾体直接刺激c-myc表达的可能性。最后，从管理的c-Myc抑制剂的结果表明，c-Myc在20 E诱导的细胞增殖中起着至关重要的作用。这些发现表明昆虫蜕皮激素诱导的细胞增殖的一种新途径，它很可能是保守的昆虫和哺乳动物之间的类固醇激素调节。(C)2015爱思唯尔有限公司版权所有。
Developmental switching from growth to metamorphosis in imaginal primordia is an essential process of adult body planning in holometabolous insects. Although it is disciplined by a sequential action of the ecdysteroid, molecular mechanisms linking to cell proliferation are poorly understood. In the present study, we investigated the expression control of cell cycle related genes by the ecdysteroid using the wing disc of the final-instar larvae of the silkworm, Bombyx mori. We found that the expression level of c-myc was remarkably elevated in the post-feeding cell proliferation phase, which coincided with a small increase in ecdysteroid titer. An in vitro wing disc culture showed that supplementation of the moderate level of the ecdysteroid upregulated c-myc expression within an hour and subsequently increased the expression of cell cycle core regulators, including A-, B-, D-, and E-type cyclin genes, Cdc25 and E2F1. We demonstrated that c-myc upregulation by the ecdysteroid was not inhibited in the presence of a protein synthesis inhibitor, suggesting a possibility that the ecdysteroid directly stimulates c-myc expression. Finally, results from the administration of a c-Myc inhibitor demonstrated that c-Myc plays an essential role in 20E-inducible cell proliferation. These findings suggested a novel pathway for ecdysteroid-inducible cell proliferation in insects, and it is likely to be conserved between insects and mammals in terms of steroid hormone regulation. (C) 2015 Elsevier Ltd. All rights reserved.