DIFFERENCES IN MODE OF PHAGOCYTOSIS WITH FC AND C3 RECEPTORS IN MACROPHAGES
DIFFERENCES IN MODE OF PHAGOCYTOSIS WITH FC AND C3 RECEPTORS IN MACROPHAGES
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DOI：
10.1111/j.1365-3083.1977.tb02153.x
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发表时间：
1977-01-01
影响因子：
3.7
通讯作者：
KAPLAN, G
中科院分区：
文献类型：
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作者：
KAPLAN, G
使体内或体外活化的小鼠巨噬细胞内化用IG[免疫球蛋白]G或IgM和补体[C]调理的绵羊红细胞[E]。在将培养物从有利于附着的条件转移到有利于内化的条件后的不同时间进行扫描电子显微镜和透射电子显微镜检查。Fc和C3 [补体成分3]的受体随机分布在巨噬细胞表面上，除了最外周，Fc受体更丰富。E-IgG被从巨噬细胞表面升起的薄膜延伸物摄取，并将调理颗粒紧紧包围在从巨噬细胞表面突出的杯状结构中。只有在这之后，被覆盖的颗粒才被吸入细胞体。E-IgMC直接沉入巨噬细胞胞质中，没有明显的膜延伸参与。细胞松弛素B的实验表明，微丝是必不可少的Fc的吞噬作用，但重要得多的C3受体。
Mouse macrophages, activated in vivo or in vitro, were made to internalize sheep erythrocytes [E] opsonized with Ig[immunoglobulin]G or IgM and complement [C]. Scanning electron microscopy and transmission electron microscopy were performed at various times after the transfer of the cultures from conditions favoring attachment to conditions favoring internalization. The receptors for Fc and C3 [complement component 3] were distributed randomly over the macrophage surface with the exception of the extreme periphery, were Fc receptors were more abundant. The E-IgG were ingested by thin membrane extensions rising from the macrophage surface and enclosing the opsonized particles tightly in a cup-like structure protruding from the macrophage surface. Only afterwards were the covered particles drawn into the cell body proper. The E-IgMC sank directly into the macrophage cytoplasm without apparent involvement of membrane extensions. Experiments with cytochalasin B suggested that microfilaments were essential for the phagocytosis by Fc but much less important with the C3 receptor.