Wide dissemination of OXA-23-producing carbapenem-resistant Acinetobacter baumannii clonal complex 22 in multiple cities of China
Wide dissemination of OXA-23-producing carbapenem-resistant Acinetobacter baumannii clonal complex 22 in multiple cities of China
复制标题
产OXA-23的耐碳青霉烯类鲍曼不动杆菌克隆复合物22在中国多个城市广泛传播
DOI：
10.1093/jac/dkq027
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发表时间：
2010-04-01
影响因子：
5.2
通讯作者：
Li, Lanjuan
中科院分区：
文献类型：
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作者：
Fu, Yiqi;Zhou, Jianying;Li, Lanjuan
本研究采用多位点序列分型（MLST）对中国多个城市耐碳青霉烯不动杆菌鲍曼不动杆菌（CRAB）和敏感鲍曼不动杆菌（CSAB）的遗传背景进行了分析。选取来自中国16个城市的152株CRAB和74株CSAB进行MLST分子鉴定。eBURST用于将序列类型（STs）聚类到克隆复合体（CCs）中，并推断进化血统。PCR检测碳青霉烯酶编码基因和上游元件为ISAba1的bla（AmpC）。CSAB比CRAB有更多的遗传背景，CSAB鉴定出36个不同的STs，而CRAB只鉴定出8个。ST22及其3个单位点变异均聚集在CC22中，是最突出的STs，占螃蟹的86.8%和CSAB的45.9%，分布在所有16个城市，具有比其他STs更明显的抗生素耐药性。大多数CRAB菌株的bla(OXA-23) PCR扩增阳性，而CSAB菌株的bla(OXA-23) PCR扩增阴性。在螃蟹的不同STs中，bla（AmpC）上游的ISAba1的存在是不同的。eBURST显示，CC22是Pubmlst数据库中最大的群，该数据库还包含先前在欧洲克隆II分离物中发现的作为CC22亚群成员的ST6。我们描述了CRAB CC22在中国的广泛传播。CC22与欧洲克隆II的亲缘关系表明CC22可能在全球传播。推断ST22-CSAB通过获得bla（OXA-23）作为决定因子进化为ST22-CRAB。
In this study, multilocus sequence typing (MLST) was used to describe the genetic backgrounds of carbapenem-resistant Acinetobacter baumannii (CRAB) and carbapenem-susceptible A. baumannii (CSAB) from multiple cities of China.One hundred and fifty-two CRAB and 74 CSAB isolates obtained from 16 cities of China were selected for molecular characterization by MLST. eBURST was used to cluster sequence types (STs) into clonal complex (CCs) and infer evolutionary descent. PCR was used to detect carbapenemase-encoding genes and bla(AmpC) with the upstream element ISAba1.CSAB showed more diverse genetic backgrounds than CRAB since 36 distinct STs were identified in CSAB while only 8 STs were identified in CRAB. ST22 and its three single-locus variants, all clustered into CC22, were the most prominent STs, accounting for 86.8% of CRAB and 45.9% of CSAB, distributed in all 16 cities and possessing more noticeable antibiotic resistance than other STs. PCR amplification was positive for bla(OXA-23) in most CRAB isolates but negative in CSAB isolates. The presence of ISAba1 upstream of bla(AmpC) was variable in distinct STs of CRAB. eBURST reveals that CC22 is the largest group in the Pubmlst database, which also contains ST6 previously identified in a European clone II isolate as a member of a subgroup of CC22.We describe the wide dissemination of CRAB CC22 in China. The close relatedness between CC22 and European clone II implies the probable global spread of CC22. It is inferred that ST22-CSAB evolves to ST22-CRAB through acquiring bla(OXA-23) as a determinative factor.