Diversity of Antimicrobial Resistance Phenotypes in Salmonella Isolated from Commercial Poultry Farms.
Diversity of Antimicrobial Resistance Phenotypes in Salmonella Isolated from Commercial Poultry Farms.
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DOI：
10.3389/fvets.2017.00096
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发表时间：
2017
影响因子：
3.2
通讯作者：
Maurer JJ
中科院分区：
文献类型：
--
作者：
Liljebjelke KA;Hofacre CL;White DG;Ayers S;Lee MD;Maurer JJ
沙门氏菌仍然是美国食源性疾病的主要原因，耐药沙门氏菌通过食物链的传播对沙门氏菌病的治疗失败具有重要意义。我们调查了沙门氏菌在综合肉鸡生产中的生态学，以了解该系统中抗生素敏感和耐药菌株的流动。数据分析来自一项回顾性研究，该研究的重点是美国FDA通过国家抗菌素耐药性监测系统（NARMS）进入零售肉类监测的最初几年期间从商业肉鸡养殖场回收的抗菌素耐药性沙门氏菌。63%的沙门氏菌对NARMS计划使用的19种抗菌剂的面板是泛敏感的。从两个肉鸡养殖场分离的沙门氏菌中观察到25种耐药表型。然而，沙门氏菌对链霉素单独耐药以及与其他抗生素联合耐药是观察到的最普遍（36.3%）的抗菌药物耐药表型。对链霉素和磺胺二甲氧嘧啶的抗性似乎与转座子Tn21有关。脉冲场凝胶电泳确定了多种肠道沙门氏菌血清型和遗传类型，观察到对链霉素、庆大霉素、磺胺二甲氧嘧啶、甲氧苄啶和四环素的耐药性组合。农场内部和农场之间沙门氏菌的抗生素敏感性存在差异，其中一些差异与特定血清型有关。然而，农场差异与抗生素使用无关。对这些养殖场流行性沙门氏菌血清型的时空分布的分析表明，防止耐药沙门氏菌的垂直传播将减少屠体受耐药沙门氏菌污染，从而减少人类暴露风险。
Salmonella remains the leading cause of foodborne illness in the United States, and the dissemination of drug-resistant Salmonellae through the food chain has important implications for treatment failure of salmonellosis. We investigated the ecology of Salmonella in integrated broiler production in order to understand the flow of antibiotic susceptible and resistant strains within this system. Data were analyzed from a retrospective study focused on antimicrobial resistant Salmonella recovered from commercial broiler chicken farms conducted during the initial years of the US FDA’s foray into retail meat surveillance by the National Antimicrobial Resistance Monitoring System (NARMS). Sixty-three percentage of Salmonella were pan-susceptible to a panel of 19 antimicrobials used by the NARMS program. Twenty-five antimicrobial resistance phenotypes were observed in Salmonella isolated from two broiler chicken farms. However, Salmonella displaying resistance to streptomycin, alone, and in combination with other antibiotics was the most prevalent (36.3%) antimicrobial resistance phenotype observed. Resistance to streptomycin and sulfadimethoxine appeared to be linked to the transposon, Tn21. Combinations of resistance against streptomycin, gentamicin, sulfadimethoxine, trimethoprim, and tetracycline were observed for a variety of Salmonella enterica serovars and genetic types as defined by pulsed-field gel electrophoresis. There were within and between farm differences in the antibiotic susceptibilities of Salmonella and some of these differences were linked to specific serovars. However, farm differences were not linked to antibiotic usage. Analysis of the temporal and spatial distribution of the endemic Salmonella serovars on these farms suggests that preventing vertical transmission of antibiotic-resistant Salmonella would reduce carcass contamination with antibiotic-resistant Salmonella and subsequently human risk exposure.