Calcium-binding protein S100P is highly expressed during the implantation window in human endometrium
Calcium-binding protein S100P is highly expressed during the implantation window in human endometrium
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钙结合蛋白S100P在人子宫内膜着床窗口期高表达
DOI：
10.1016/j.fertnstert.2009.07.1667
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发表时间：
2010-09-01
影响因子：
6.7
通讯作者：
Zhang, Song-ying
中科院分区：
文献类型：
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作者：
Tong, Xiao-Mei;Lin, Xiao-Na;Zhang, Song-ying
目的：研究S100 P在人子宫内膜中的表达和定位，在整个月经周期中，设计：实验研究，设置：大学医院，患者：84名妇女，干预：对LH+4、LH+7和hCG+7天的人子宫内膜进行互补DNA（cDNA）微阵列分析。逆转录聚合酶链反应（RT-PCR）和蛋白质印迹分析用于检测S100 P和其他S100家族成员S100 A4，S100 A13和S100 A6的表达。采用免疫荧光法检测S100 P蛋白在LH+7和LH+4子宫内膜中的定位。主要观察指标：差异基因表达、S100 P信使RNA（mRNA）水平、S100 P蛋白表达和免疫荧光定位。我们发现，S100 P是第四个最上调的基因在LH+7。S100 P的mRNA和蛋白水平在增殖期和LH+4时相当低，但在LH+7时显著升高。hCG+7时S100 P表达低于LH+7时。S100 A4、S100 A13和S100 A6的表达在整个月经周期中没有变化。S100 P的潜在生物学效应需要进一步探索。（Fertil Steril（R）2010;94：1510-8. (C)美国生殖医学会（American Society for Reproductive Medicine）
Objective: To investigate S100P expression and localization in human endometrium throughout the menstrual cycle.Design: Experimental study.Setting: University hospital.Patient(s): Eighty-four women.Intervention(s): Complementary DNA (cDNA) microarray analysis was performed on human endometrium from days LH+4, LH+7, and hCG+7. Reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analysis were used to detect the expression of S100P and of additional S100 family members, S100A4, S100A13, and S100A6. Immunofluorescence was used to detect the localization of S100P protein in LH+7 and LH+4 endometrium.Main Outcome Measure(s): Differential gene expression, levels of S100P messenger RNA (mRNA), and protein expression and immunofluorescent localization of S100P.Result(s): A statistical method, based on hierarchical clustering, identified genes whose expression varied at LH+7 compared with LH+4. We found that S100P was the fourth most up-regulated gene at LH+7. The S100P mRNA and protein levels were quite low during the proliferative phase and LH+4, but were elevated significantly at LH+7. The S100P expression at hCG+7 was lower than that at LH+7. However, the expression of S100A4, S100A13, and S100A6 did not vary throughout the menstrual cycle.Conclusion(s): S100P was specifically up-regulated during the implantation window. The underlying biological effects of S100P need further exploration. (Fertil Steril (R) 2010;94:1510-8. (C) 2010 by American Society for Reproductive Medicine.)