Genipin crosslinked bioactive collagen/chitosan/hyaluronic acid injectable hydrogels structurally amended via covalent attachment of surface-modified silica particles
Genipin crosslinked bioactive collagen/chitosan/hyaluronic acid injectable hydrogels structurally amended via covalent attachment of surface-modified silica particles
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DOI：
10.1016/j.ijbiomac.2019.06.184
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发表时间：
2019-09-01
影响因子：
8.2
通讯作者：
Nowakowska, Maria
中科院分区：
文献类型：
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作者：
Lewandowska-Lancucka, Joanna;Gilarska, Adriana;Nowakowska, Maria
以胶原蛋白、壳聚糖和透明质酸为基础的用于骨组织工程应用的多组分可注射且原位凝胶化仿生杂化材料通过一步法制备。将表面改性的二氧化硅颗粒形式的生物活性相引入生物聚合物溶液中，并在37℃下同时用京尼平使生物聚合物基质和分散的颗粒交联。这里提出的新方法涉及使用表面预先用氨基官能化的二氧化硅颗粒。这种改性使得在与京尼平交联时二氧化硅颗粒能够共价连接到聚合物水凝胶网络上。这种方法特别重要，因为它能够获得杂化材料（生物聚合物 - 二氧化硅颗粒），其中与矿物颗粒潜在相分离相关的问题（阻碍其体内应用）可以被消除。获得了各种组成的杂化物，并确定了它们的物理化学和生物学特性。在模拟体液条件下进行的体外实验表明，共价连接到生物聚合物网络的氨基官能化二氧化硅颗粒仍然具有生物活性。最后，体外细胞培养研究表明，所开发的材料具有生物相容性，因为它们支持MG - 63细胞的粘附、增殖以及碱性磷酸酶（ALP）的表达。（C）2019爱思唯尔有限公司保留所有权利。
Collagen, chitosan and hyaluronic acid based multicomponent injectable and in situ gellating biomimetic hybrid materials for bone tissue engineering applications were prepared in one-step procedure. The bioactive phase in the form of surface-modified silica particles was introduced to the solutions of biopolymers and simultaneously crosslinked with genipin both the biopolymer matrix and dispersed particles at 37 degrees C. The novel approach presented here involved the use of silica particles which surfaces were priory functionalized with amino groups. That modification makes possible the covalent attachment of silica particles to the polymeric hydrogel network on crosslinking with genipin. That methodology is especially important as it makes possible to obtain the hybrid materials (biopolymer-silica particles) in which the problems related to the potential phase separation of mineral particles, hindering their in vivo application can be eliminated. The hybrids of various compositions were obtained and their physicochemical and biological properties were determined. The in vitro experiments performed under simulated body fluid conditions revealed that the amino-functionalized silica particles covalently attached to the biopolymeric network are still bioactive. Finally, the in vitro cell culture studies shown that the materials developed are biocompatible as they supported MG-63 cells adhesion, proliferation as well as Alkaline phosphatase (ALP) expression. (C) 2019 Elsevier B.V. All rights reserved.