P. aeruginosa in the paranasal sinuses and transplanted lungs have similar adaptive mutations as isolates from chronically infected CF lungs
P. aeruginosa in the paranasal sinuses and transplanted lungs have similar adaptive mutations as isolates from chronically infected CF lungs
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DOI：
10.1016/j.jcf.2013.02.004
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发表时间：
2013-12-01
影响因子：
5.2
通讯作者：
Hoiby, Niels
中科院分区：
文献类型：
--
作者：
Ciofu, Oana;Johansen, Helle Krogh;Hoiby, Niels
工作背景：铜绿假单胞菌细胞作为生物膜存在于慢性感染的囊性纤维化（CF）患者的鼻窦和肺中。由于与肺部相比，鼻窦中的炎症反应和选择性抗生素压力不同，因此我们比较了来自两个位置的粘液和非粘液分离株的适应性特征。我们研究了从4名慢性感染CF患者中分离的肺和鼻窦铜绿假单胞菌的表型多样性和基因表达谱的遗传基础，结果：从鼻窦和肺移植前后分离的粘液型和非粘液型分离株中发现了由相似的突变和相似的基因表达谱引起的相同的表型。慢性感染患者的副鼻窦和肺之间的铜绿假单胞菌分离株的双侧交换发生在肺移植前，广泛的鼻窦手术可能会防止新肺的感染。(C)2013年欧洲囊性纤维化协会。Elsevier B.V.出版，保留所有权利。
Background: Pseudomonas aeruginosa cells are present as biofilms in the paranasal sinuses and the lungs of chronically infected cystic fibrosis (CF) patients. Since different inflammatory responses and selective antibiotic pressures are acting in the sinuses compared with the lungs, we compared the adaptive profiles of mucoid and non-mucoid isolates from the two locations.Methods: We studied the genetic basis of phenotypic diversification and gene expression profiles in sequential lung and sinus P. aeruginosa isolates from four chronically infected CF patients, including pre- and post-lung transplantation isolates.Results: The same phenotypes caused by similar mutations and similar gene expression profiles were found in mucoid and non-mucoid isolates from the paranasal sinuses and from the lungs before and after transplantation.Conclusion: Bilateral exchange of P. aeruginosa isolates between the paranasal sinuses and the lungs occurs in chronically infected patients and extensive sinus surgery before the lung transplantation might prevent infection of the new lung. (C) 2013 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.