Taenia solium and Taenia crassiceps: miRNomes of the larvae and effects of miR-10-5p and let-7-5p on murine peritoneal macrophages
Taenia solium and Taenia crassiceps: miRNomes of the larvae and effects of miR-10-5p and let-7-5p on murine peritoneal macrophages
复制标题
DOI：
10.1042/bsr20190152
复制
发表时间：
2019-11-19
影响因子：
4
通讯作者：
Jimenez, Lucia
中科院分区：
文献类型：
--
作者：
Landa, Abraham;Navarro, Luz;Jimenez, Lucia
神经囊虫病（NCC）是世界范围内神经系统疾病的主要原因，由猪带绦虫幼虫引起。寄生蜂分泌的分子可以阻断宿主的Th1反应，并诱导允许其建立的Th2反应。成熟的microrna （miRs）是一种小的非编码rna，它调节基因表达并参与免疫过程。为了确定带绦虫miRs在囊尾蚴免疫应答中的作用，我们从猪带绦虫和带绦虫的幼虫中构建了小RNA （sRNA）文库。分别有12074504条和11779456条测序reads被定位到钉螺和其他蠕虫的基因组中。两种幼虫具有相似的miRNome， miR-10-5p在两种中含量最高，其次是t-7-5p和t- 4989-3p，而在属特异性miRs中，miR-001-3p在两种中含量最高，其次是t- solium中的mir -002-3p和t- assiceps中的miR-003a-3p。这两个mir的序列是相同的。结构和靶标预测分析显示，这些pre- mir形成一个发夹，并且有多个参与免疫调节的靶标。巨噬细胞培养、RT-PCR和ELISA检测显示，细胞内化miR-10-5p和让mir -7-5p进入细胞质，miR-10-5p强烈降低白细胞介素16 （Il6）表达、肿瘤坏死因子（TNF）和IL-12分泌，适度降低M（IL-4）巨噬细胞中一氧化氮合酶诱导（Nos2）和il - 1b（促炎因子）表达、Tgf1b表达和IL-10（抗炎因子）分泌。这些发现可以帮助我们理解mir在宿主-带绦虫关系中的作用。
Neurocysticercosis (NCC), a major cause of neurological morbidity worldwide, is caused by the larvae of Taenia solium. Cestodes secrete molecules that block the Th1 response of their hosts and induce a Th2 response permissive to their establishment. Mature microRNAs (miRs) are small noncoding RNAs that regulate gene expression and participate in immunological processes. To determine the participation of Taenia miRs in the immune response against cysticercosis, we constructed small RNA (sRNA) libraries from larvae of Taenia solium and Taenia crassiceps. A total of 12074504 and 11779456 sequencing reads for T. solium and T. crassiceps, respectively, were mapped to the genomes of T. solium and other helminths. Both larvae shared similar miRNome, and miR-10-5p was the most abundant in both species, followed by let-7-5p in T. solium and miR-4989-3p in T. crassiceps, whereas among the genus-specific miRs, miR-001-3p was the most abundant in both, followed bymiR-002-3p in T. solium and miR-003a-3p in T. crassiceps. The sequences of these miRs were identical in both. Structure and target prediction analyses revealed that these pre-miRs formed a hairpin and had more than one target involved in immunoregulation. Culture of macrophages, RT-PCR and ELISA assays showed that cells internalized miR-10-5p and let-7-5p into the cytoplasm and the miRs strongly decreased interleukin 16 (Il6) expression, tumor necrosis factor (TNF) and IL-12 secretion, and moderately decreased nitric oxide synthase inducible (Nos2) and Il1b expression (pro-inflammatory cytokines) in M(IFN-gamma) macrophages and expression of Tgf1b, and the secretion of IL-10 (anti-inflammatory cytokines) in M(IL-4) macrophages. These findings could help us understand the role of miRs in the host-Taenia relationship.