Assembly of Tetraspanin-enriched macrodomains contains membrane damage to facilitate repair
Assembly of Tetraspanin-enriched macrodomains contains membrane damage to facilitate repair
复制标题
富含四跨膜蛋白的宏结构域的组装包含膜损伤以促进修复
DOI：
10.1038/s41556-022-00920-0
复制
发表时间：
2022-06-02
影响因子：
21.3
通讯作者：
Yu, Li
中科院分区：
文献类型：
--
作者：
Huang, Yuwei;Zhang, Xing;Yu, Li
各种机制有助于膜修复（1-3），但介导质膜上大伤口修复的机制尚不清楚。我们发现，膜损伤后不久，四跨膜蛋白富集的宏域组装在损伤部位周围。富含四跨膜蛋白的大结构域处于液体有序相，并在受损部位周围形成刚性环。这限制了损伤的扩散并防止膜崩解，从而通过其他机制促进膜修复。在功能上，Tetraspanin 4帮助细胞减轻激光，清洁剂，pyroptosis和自然杀伤细胞造成的损伤。我们认为四跨膜蛋白富集的大结构域的组装产生了一个物理屏障来抑制膜损伤。
Various mechanisms contribute to membrane repair(1-3) but the machinery that mediates the repair of large wounds on the plasma membrane is less clear. We found that shortly after membrane damage, Tetraspanin-enriched macrodomains are assembled around the damage site. Tetraspanin-enriched macrodomains are in the liquid-ordered phase and form a rigid ring around the damaged site. This restricts the spread of the damage and prevents membrane disintegration, thus facilitating membrane repair by other mechanisms. Functionally, Tetraspanin 4 helps cells mitigate damage caused by laser, detergent, pyroptosis and natural killer cells. We propose that assembly of Tetraspanin-enriched macrodomains creates a physical barrier to contain membrane damage.