Identification of melanoma antigens using a Serological Proteome Approach (SERPA).
Identification of melanoma antigens using a Serological Proteome Approach (SERPA).
复制标题
DOI：
--
复制
发表时间：
2010
影响因子：
2.5
通讯作者：
A. Suzuki;A. Iizuka;Masaru Komiyama;Masako Takikawa;A. Kume;Sachiko Tai;Chie Ohshita;Ayumi Kurusu-Ayumi-Kur
中科院分区：
文献类型：
--
作者：
A. Suzuki;A. Iizuka;Masaru Komiyama;Masako Takikawa;A. Kume;Sachiko Tai;Chie Ohshita;Ayumi Kurusu-Ayumi-Kur
黑色素瘤是一种难治性癌症，预后差，在世界范围内发病率不断增加。特异性生物标志物的早期diagnosis. MATERIALS和METHODS黑色素瘤患者和健康志愿者的血清样品被用于识别黑色素瘤标志物蛋白质，使用血清学蛋白质组学方法。具体地，将通过二维凝胶电泳分离并转移到膜上的G361细胞蛋白质点与患者血清一起孵育，并使用飞行时间质谱法鉴定与5个以上血清样品反应的阳性点。结果G361凝胶上只有病人血清有多个斑点反应。共检测到13个阳性斑点，鉴定出5个蛋白：真核细胞延伸因子2（EEF 2）、烯醇化酶1（ENO 1）、醛缩酶A（ALDOA）、甘油醛-3-磷酸脱氢酶（GAPDH）和异质核核糖核蛋白（HNRNP）A2 B1。与黑素细胞相比，4种蛋白质（EEF 2、ENO 1、ALDOA和HNRNPA 2B 1）的mRNA在G361细胞中高表达。EEF 2、ENO 1和ALDOA mRNA也在其他黑素瘤细胞系中频繁表达。结论基于自身抗体的蛋白质组学方法是研究黑色素瘤生物标志物的有效方法。这项研究可能有助于开发早期检测癌症的诊断设备。
BACKGROUND Melanoma is an intractable cancer with a poor prognosis and increasing prevalence worldwide. Specific biomarkers for early diagnosis have yet to be found. MATERIALS AND METHODS Serum samples from melanoma patients and healthy volunteers were utilized for identifying melanoma marker proteins using a serological proteome approach. Specifically, G361 cell protein spots separated by 2-dimensional gel electrophoresis and transferred to a membrane were incubated with patient sera, and positive spots that reacted with more than 5 serum samples were identified using time of flight mass spectrometry. RESULTS Only patient sera showed many spots reacted in G361 gels. A total of 13 positive spots were detected and 5 proteins were identified: eukaryotic elongation factor2 (EEF2), enolase1 (ENO1), aldolase A (ALDOA), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and heterogeneous nuclear ribonucleoproteins (HNRNP) A2B1. The mRNAs of four proteins (EEF2, ENO1, ALDOA and HNRNPA2B1) were highly expressed in G361 cells compared with melanocytes. EEF2, ENO1 and ALDOA mRNAs were also frequently expressed in other melanoma cell lines. CONCLUSION The autoantibody-based proteomic approach was effective for investigating melanoma biomarkers. This study might contribute to the development of a diagnostic device for the early detection of cancer.