Increase in IS256 transposition in invasive vancomycin heteroresistant Staphylococcus aureus isolate belonging to ST100 and its derived VISA mutants
Increase in IS256 transposition in invasive vancomycin heteroresistant Staphylococcus aureus isolate belonging to ST100 and its derived VISA mutants
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DOI：
10.1016/j.meegid.2016.05.001
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发表时间：
2016-09-01
影响因子：
3.2
通讯作者：
Mollerach, Marta
中科院分区：
文献类型：
--
作者：
Di Gregorio, Sabrina;Fernandez, Silvina;Mollerach, Marta
在金黄色葡萄球菌中，IS256的转座已被描述为在生物膜形成和抗生素耐药性中起重要作用。本研究描述了两种临床异质性万古霉素中间体S的分子特征。金黄色葡萄球菌（hVISA）分离物从同一患者（抗生素治疗之前和之后）回收，并且两种VISA衍生物在万古霉素存在下通过连续传代获得。我们的研究结果表明，抗生素治疗（在体内和体外）可以增强IS256转座，负责最终失去agr功能。据我们所知，这是第一个研究，报告增加IS256转座后，在临床环境中抗生素治疗的同基因菌株。(C)2016爱思唯尔B.V.保留所有权利。
In Staphylococcus aureus, transposition of IS256 has been described to play an important role in biofilm formation and antibiotic resistance. This study describes the molecular characterization of two clinical heterogeneous vancomycin-intermediate S. aureus (hVISA) isolates recovered from the same patient (before and after antibiotic treatment) and two VISA derivatives obtained by serial passages in the presence of vancomycin. Our results showed that antibiotic treatment (in vivo and in vitro) could enhance IS256 transposition, being responsible for the eventual loss of agr function. As far as we know this is the first study that reports the increase of IS256 transposition in isogenic strains after antibiotic treatment in a clinical setting. (C) 2016 Elsevier B.V. All rights reserved.