HLA‐D Restriction of the Macrophage‐Dependent Response of Immune Human T Lymphocytes to PPD in Vitro: Inhibition by Anti‐HLA‐DR Antisera
HLA‐D Restriction of the Macrophage‐Dependent Response of Immune Human T Lymphocytes to PPD in Vitro: Inhibition by Anti‐HLA‐DR Antisera
复制标题
HLA-D 限制免疫人 T 淋巴细胞对体外 PPD 的巨噬细胞依赖性反应：抗 HLA-DR 抗血清的抑制
DOI：
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发表时间：
1978
影响因子：
3.7
通讯作者：
Erik Thorsby
中科院分区：
文献类型：
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作者：
B. Bergholtz;Erik Thorsby
体外实验中，致敏个体的T细胞对低剂量PPD的反应是巨噬细胞依赖性的。通过测试巨噬细胞和T淋巴细胞的不同异体组合，研究人员发现，最佳应答要求抗原由巨噬细胞呈递，这些巨噬细胞至少共享T细胞供体的HLA - D决定因子之一。发现识别HLA - A， - B或- DR抗原的抗血清能够抑制这种增殖反应。研究发现，抗HLA - DR抗血清仅在针对T淋巴细胞和巨噬细胞供体共有的抗原时才发挥抑制作用。然而，抗HLA - A和- B血清在与应答的T淋巴细胞反应时具有抑制作用，而不管它们与巨噬细胞的反应性如何。由此得出结论，体内免疫T淋巴细胞对PPD的最佳二次应答需要抗原和HLA - D基因座编码的“自身”膜结构的联合识别。
The response of T cells from sensitized individuals to low doses of PPD in vitro is macrophage‐dependent. By testing different allogeneic combinations of macrophages and T lymphocytes, it was found that an optimal response required that antigen be presented by macrophages sharing at least one of the HLA‐D determinants of the T cell donor. Antisera recognizing HLA‐A, ‐B or ‐DR antigens were found to be able to inhibit this proliferative response. The anti‐HLA‐DR antisera were found to exert their inhibitory effect only when directed towards an antigen shared by the donors of the T lymphocytes and the macrophages. Anti‐HLA‐A and ‐B sera, however, had an inhibitory effect when reactive with the responding T lymphocytes, irrespective of their reactivity with the cooperating macrophages. It is concluded that an optimal secondary response of in‐vivo‐immunized T lymphocytes to PPD requires the combined recognition of the antigen and ‘self’ membrane structures encoded by the HLA‐D locus.