Plasma microRNA-451 as a novel hemolytic marker for β0-thalassemia/HbE disease.
Plasma microRNA-451 as a novel hemolytic marker for β0-thalassemia/HbE disease.
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DOI：
10.3892/mmr.2017.6326
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发表时间：
2017-05
影响因子：
3.4
通讯作者：
Umemura T
中科院分区：
文献类型：
--
作者：
Leecharoenkiat K;Tanaka Y;Harada Y;Chaichompoo P;Sarakul O;Abe Y;Smith DR;Fucharoen S;Svasti S;Umemura T
在东南亚，特别是在泰国，β0-地中海贫血/血红蛋白E（HbE）疾病是一种常见的遗传性血液病。它与病理生理过程有关，如未成熟红细胞的髓内破坏和成熟红细胞的外周溶血。microRNA（miR）序列是以抑制方式调节基因表达的短的非编码RNA，在人类红细胞生成中起关键作用。在本研究中，分析了23名β0-地中海贫血/HbE患者和16名对照受试者的红细胞表达的miRNA（miR-451和miR-155）的血浆水平。逆转录-定量聚合酶链反应分析显示，与对照受试者相比，β0-地中海贫血/HbE患者的血浆miR-451和miR-155水平显著更高。值得注意的是，在β0-地中海贫血/HbE患者中，与轻度病例相比，在重度病例中检测到miR-451水平显著增加。血浆miR-451水平与网织红细胞和血小板计数相关。结果表明，血浆miR-451水平升高可能与β0-地中海贫血/HbE患者的溶血程度和红细胞生成加速相关。总之，miR-451可能代表与β0-地中海贫血/HbE相关的病理性红细胞生成的相关生物标志物。
In Southeast Asia, particularly in Thailand, β0-thalassemia/hemoglobin E (HbE) disease is a common hereditary hematological disease. It is associated with pathophysiological processes, such as the intramedullary destruction of immature erythroid cells and peripheral hemolysis of mature red blood cells. MicroRNA (miR) sequences, which are short non-coding RNA that regulate gene expression in a suppressive manner, serve a crucial role in human erythropoiesis. In the present study, the plasma levels of the erythroid-expressed miRNAs, miR-451 and miR-155, were analyzed in 23 patients with β0-thalassemia/HbE and 16 control subjects. Reverse transcription-quantitative polymerase chain reaction analysis revealed significantly higher levels of plasma miR-451 and miR-155 in β0-thalassemia/HbE patients when compared to the control subjects. Notably, among the β0-thalassemia/HbE patients, a significant increase in miR-451 levels was detected in severe cases when compared with mild cases. The levels of plasma miR-451 correlated with reticulocyte and platelet counts. The results suggest that increased plasma miR-451 levels may be associated with the degree of hemolysis and accelerated erythropoiesis in β0-thalassemia/HbE patients. In conclusion, miR-451 may represent a relevant biomarker for pathological erythropoiesis associated with β0-thalassemia/HbE.