Tumor necrosis factor and interleukin-1 stimulate testosterone secretion in adult male rat Leydig cells in vitro.
Tumor necrosis factor and interleukin-1 stimulate testosterone secretion in adult male rat Leydig cells in vitro.
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发表时间：
1990-07
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通讯作者：
Dwight W. Warren;V. Pasupuleti;Yin Lu;B. W. Platler;R. Horton
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作者：
Dwight W. Warren;V. Pasupuleti;Yin Lu;B. W. Platler;R. Horton
研究了肿瘤坏死因子(TNF)和白介素1(IL-1)两种细胞因子对分散的成人睾丸细胞和纯化的间质细胞产生睾酮的影响。在一组实验中，成年(90天龄)大鼠的睾丸细胞被酶法分散。在另一组实验中，将分散的睾丸细胞放置在Percoll密度梯度上，然后离心以获得纯化的(大于85%)间质细胞。将整个睾丸细胞和纯化的间质细胞分别加入不同剂量的肿瘤坏死因子或白介素1和最大刺激量的人绒毛膜促性腺激素(HCG)。肿瘤坏死因子和白介素1均能促进整个睾丸细胞和纯化的间质细胞睾酮的基础分泌。此外，肿瘤坏死因子和白介素1都能最大限度地增加hCG刺激的睾酮分泌。这两种细胞因子都能刺激分散的睾丸细胞分泌睾酮，最早刺激4小时，持续到72小时。这些细胞因子在24小时后轻微但显著地刺激纯化的间质细胞产生睾酮，并持续到72小时。由此我们得出结论，肿瘤坏死因子和白介素1可刺激成年大鼠睾丸间质细胞分泌睾酮。虽然这种作用可能是通过细胞因子对睾丸巨噬细胞的作用而介导的，但也可能对睾丸间质细胞产生直接影响，因为纯化的间质细胞以及整个睾丸细胞的分泌增加。因此，肿瘤坏死因子和白介素1可能是间质细胞功能的局部调节因子。
The actions of two cytokines, tumor necrosis factor (TNF) and interleukin-1 (IL-1), on testosterone production by dispersed adult testis cells and purified Leydig cells in culture were studied. In one set of experiments, testis cells from adult (90-day-old) rats were enzymatically dispersed. In another set of experiments, the dispersed testis cells were placed on a Percoll density gradient and were centrifuged to yield purified (greater than 85%) Leydig cells. Both whole testis cells and purified Leydig cells were cultured in the presence of varying doses of TNF or IL-1 with or without maximally stimulating doses of human chorionic gonadotropin (hCG). Both TNF and IL-1 stimulated basal secretion of testosterone in whole testis cells, as well as purified Leydig cells. Additionally, both TNF and IL-1 augmented maximally hCG stimulated testosterone secretion. Both cytokines stimulated testosterone secretion by dispersed testis cells as early as 4 hours, and the effect continued for up to 72 hours. The cytokines slightly, but significantly, stimulated testosterone production in purified Leydig cells after 24 hours, and continued for up to 72 hours. We have concluded from this data that TNF and IL-1 stimulate the testosterone secretion by adult rat Leydig cells. While this effect might be mediated through the action of the cytokines on testicular macrophages, there might also be a direct effect on the Leydig cell since augmentation of secretion occurred in purified Leydig cells, as well as whole testis cells. Therefore, TNF and IL-1 may serve as local regulators of Leydig cell function.