Bacillibactin-mediated iron transport in Bacillus subtilis
Bacillibactin-mediated iron transport in Bacillus subtilis
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DOI：
10.1021/ja055898c
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发表时间：
2006-01-11
影响因子：
15
通讯作者：
Raymond, KN
中科院分区：
文献类型：
--
作者：
Dertz, EA;Xu, JD;Raymond, KN
枯草芽孢杆菌利用两个部分重叠的膜(1和2)从其内源铁载体(杆菌和衣酸)中获取铁。肠杆菌蛋白和杆状杆菌蛋白具有相反的金属手性，对铁离子的亲和力不同，铁的转运行为也不同。用电位法和分光光度滴定法研究了杆菌素铁溶液的热力学稳定性。在邻苯二酚螯合臂上添加甘氨酸会导致杆状杆菌蛋白的铁复合体的不稳定。枯草杆菌似乎表达一个单独的肠杆菌素受体(渗透酶3)，尽管肠杆菌素也可以通过杆状杆菌蛋白的渗透酶(渗透酶2)运输。
The hexadentate triscatecholamide bacillibactin delivers iron toBacillus subtilisand is structurally similar to enterobactin, although in a more oblate conformation.B. subtilisuses two partially overlapping permeases (1 and 2) to acquire iron from its endogenous siderophores (bacillibactin and itoic acid). Enterobactin and bacillibactin have opposite metal chiralities, different affinity for ferric ion, and dissimilar iron transport behaviors. The solution thermodynamic stability of ferric bacillibactin has been investigated through potentiometric and spectrophotometric titrations. The addition of a glycine to the catechol chelating arms causes a destabilization of the ferric complex of bacillibactin compared to ferric enterobactin.B. subtilisappears to express a separate receptor for enterobactin (permease 3), although enterobactin can also be transported through the permease for bacillibactin (permease 2).