NUCLEAR TRANSPLANTATION IN THE MOUSE EMBRYO BY MICROSURGERY AND CELL-FUSION
NUCLEAR TRANSPLANTATION IN THE MOUSE EMBRYO BY MICROSURGERY AND CELL-FUSION
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DOI：
10.1126/science.6857250
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发表时间：
1983-01-01
期刊：
影响因子：
56.9
通讯作者：
SOLTER, D
中科院分区：
文献类型：
--
作者：
MCGRATH, J;SOLTER, D
小鼠胚胎的核移植是通过采用显微手术切除受精卵原核和通过病毒介导的细胞融合技术引入供体核相结合的方法实现的。在对该程序的测试中，胚胎存活率大于90%。胚胎发育到足月的频率与未处理的对照胚胎没有显著差异。由于遗传上不同的近交系小鼠的细胞核和细胞质可以有效地互换，这一过程可能有助于确定可能的细胞质对胚胎和成体表型的贡献。
Nuclear transplantation in the mouse embryo was achieved by using a method that combines microsurgical removal of the zygote pronuclei with the introduction of a donor nucleus by a virus-mediated cell fusion technique. Survival of embryos was greater than 90% in tests of this procedure. The embryos developed to term at a frequency not significantly different from that of nonmanipulated control embryos. Because nuclei and cytoplasm from genetically distinct inbred mouse strains can be efficiently interchanged, this procedure may be useful in characterizing possible cytoplasmic contributions to the embryonic and adult phenotype.