EXPRESSION OF THE 170-KDA AND 180-KDA ISOFORMS OF DNA TOPOISOMERASE-II IN RESTING AND PROLIFERATING HUMAN-LYMPHOCYTES
EXPRESSION OF THE 170-KDA AND 180-KDA ISOFORMS OF DNA TOPOISOMERASE-II IN RESTING AND PROLIFERATING HUMAN-LYMPHOCYTES
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DOI：
10.1111/j.1365-2184.1994.tb01423.x
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发表时间：
1994-05-01
影响因子：
8.5
通讯作者：
RICOTTI, GCBA
中科院分区：
文献类型：
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作者：
PROSPERI, E;NEGRI, C;RICOTTI, GCBA
在植物血凝素 (PHA) 刺激之前和之后，使用两种特异性单克隆抗体 (MoAb) 研究了人外周血淋巴细胞 (PBL) 中 DNA 拓扑异构酶 II (topo II) 的 170-kDa (α) 和 180-kDa (β) 亚型的表达。通过间接免疫荧光标记检测每种 MoAb 的结合，并通过流式细胞术进行定量。在静息 PBL 中，两种同工酶的免疫染色强度都非常低；然而，拓扑 II β 相关的免疫荧光比 或 亚型相关的免疫荧光显着高约 2.5 倍（P < 0.001）。在 PHA 刺激 48 至 72 小时之间，当发现处于 S 期和 G(2)+M 期的细胞百分比最高时，拓扑 II α 和 β 的水平分别增加至静息 PBL 中测量值的约 30 倍和 10 倍。因此，两种亚型达到了相当的免疫荧光值。在较长的刺激时间（96-120小时）下，topo II α免疫荧光没有显着变化，而相对于topo II β下降至峰值的约50%（P
The expression of the 170-kDa (alpha) and the 180-kDa (beta) isoforms of DNA topoisomerase II (topo II) was investigated with two specific monoclonal antibodies (MoAbs) in human peripheral blood lymphocytes (PBL), before and after phytohaemoagglutinin (PHA) stimulation. Binding of each MoAb was detected by indirect immunofluorescence labelling and quantified with flow cytometry. In resting PBL, the intensity of immunostaining was very low for both isozymes; however, topo II beta-associated immunofluorescence was about 2.5 times significantly higher (P < 0.001) than that associated with the or isoform. Between 48 and 72 h of PHA stimulation, when the highest percentage of cells in S and G(2)+M phases was found, the levels of topo II alpha and beta increased up to about 30 and 10 times the value measured in resting PBL, respectively. Thus, the two isoforms reached comparable immunofluorescence values. At longer stimulation periods (96-120 h), topo II alpha immunofluorescence was not significantly changed, while that relative to topo II beta declined to about 50% of the peak value (P