Evaluation of the safety of time-lapse observations for human embryos
Evaluation of the safety of time-lapse observations for human embryos
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DOI：
10.1007/s10815-010-9385-8
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发表时间：
2010-02-01
影响因子：
3.1
通讯作者：
Ando, Hisao
中科院分区：
文献类型：
--
作者：
Nakahara, Tatsuo;Iwase, Akira;Ando, Hisao
评估集成光学显微镜的光效应，并评估使用内置显微镜培养箱进行延时观察的安全性。我们前瞻性地比较了在集成光学显微镜培养箱中每隔15分钟延时观察的胚胎与在常规培养箱中间歇观察（每天1次）的胚胎在卵胞浆内单精子注射后的受精率和胚胎形态。受精率（57.5% vs 57.5%）和优良型卵裂胚率（36.0% vs 36.0%）差异无统计学意义。这些结果表明，使用集成光学显微镜的培养箱进行延时观察可以安全地用于临床实践。
To assess the effects of light from an integrated optical microscope and evaluate the safety of time-lapse observations using a built-in microscope incubator.We prospectively compared the fertilization rate and embryonic morphology after intracytoplasmic sperm injection between embryos cultured with time-lapse observations every 15 min in an incubator with an integrated optical microscope and embryos with intermittent observations (once a day) in conventional incubators.No significant differences were observed in the fertilization rate (57.5% vs. 57.5%) or the rate of excellent-good cleavage embryos (36.0% vs. 36.0%).These results suggest that time-lapse observations using an incubator with an integrated optical microscope may therefore be safely utilized in clinical practice.