The effect of divalent and univalent binding on antibody titration curves in solid-phase ELISA.
The effect of divalent and univalent binding on antibody titration curves in solid-phase ELISA.
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固相 ELISA 中二价和单价结合对抗体滴定曲线的影响。
DOI：
10.1016/0022-1759(87)90240-7
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发表时间：
1987
影响因子：
2.2
通讯作者：
J. Haaijman
中科院分区：
文献类型：
--
作者：
Q. Vos;E. Klasen;J. Haaijman
本文介绍了固相ELISA中抗原包被浓度、抗原分子表位密度和抗免疫球蛋白试剂对抗体滴定曲线的影响。基于用荧光素作为半抗原和单克隆抗荧光素抗体获得的结果（在另一种抗原-抗体系统中得到证实），得出以下结论：（a）抗体滴定曲线仅在有限的浓度范围内与抗原包被浓度无关。(b)一个抗体和两个表位之间的复合物（“二价结合”）比一个抗体和一个表位之间的复合物（“单价结合"）更稳定。二价和单价结合之间的比率取决于每个抗原分子的表位密度和抗原包被浓度。(c)前带现象可以解释为由于从二价到单价结合的转变，板结合抗体的不稳定性增加。(d)在固相ELISA中，只有在确定所有靶抗原都在饱和条件下包被时，才能正确评价抗血清特异性。
This paper describes the influence of antigen coating concentration, epitope density per antigen molecule and anti-immunoglobulin reagents on antibody titration curves in solid-phase ELISA. Based on results obtained with fluorescein as the hapten and monoclonal anti-fluorescein antibody, which were confirmed in another antigen-antibody system, it is concluded that:(a) Antibody titration curves are independent of antigen-coating concentration in a limited range of concentrations only.(b) The complex between one antibody and two epitopes ('divalent binding') is more stable than the complex between one antibody and one epitope ('univalent binding). The ratio between divalent and univalent binding depends on the epitope density per antigen molecule and on the antigen-coating concentration.(c) The prozone phenomenon can be explained by an increased instability of plate bound antibodies due to a shift from divalent to univalent binding.(d) In solid-phase ELISA a correct evaluation of the antiserum specificity can be performed only if it is ascertained that all target antigens are coated under saturating conditions.
人体组织中含有 IgA1、IgA2 和 J 链的细胞的分布。
DOI：
--
发表时间：
1984
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Crago,SS;Kutteh,WH;Moro,I;Allansmith,MR;Radl,J;Haaijman,JJ;Mestecky,J
通讯作者：
Mestecky,J