Effect of ambient particulate matter exposure on hemostasis
Effect of ambient particulate matter exposure on hemostasis
复制标题
DOI：
10.1016/j.trsl.2006.12.009
复制
发表时间：
2007-06-01
影响因子：
7.8
通讯作者：
Henriksen, Ruth Ann
中科院分区：
文献类型：
--
作者：
Cozzi, Emily;Wingard, Christopher J.;Henriksen, Ruth Ann
流行病学研究已经将环境空气中的颗粒物（PM）水平与心血管死亡率以及心肌梗死（MI）和中风住院率联系起来。血栓形成在增强急性心血管事件中起主要作用，本研究旨在确定肺暴露于PM是否会改变止血。从查佩尔山，NC airshed收集PM，并以先前显示加剧心肌缺血-再灌注损伤的剂量通过静脉滴注给予小鼠。暴露后24小时，循环血小板数量和血浆纤维蛋白原和可溶性P-选择素浓度增加。血浆组织因子途径抑制物（TFPI）浓度降低，血浆纤溶酶原激活物抑制物（派-1）浓度升高。与这些观察结果一致，尾尖横断的出血时间缩短。这些结果提供了证据，表明PM暴露改变了其他健康动物的止血，从而可能促进易感个体的血凝块形成并阻碍血凝块消退。结果还确定了明确的止血终点，可用于进一步研究剂量和颗粒特性对周围颗粒毒性的影响。
Epidemiological studies have linked levels of particulate matter (PM) in ambient air to cardiovascular mortality and hospitalizations for myocardial infarction (MI) and stroke. Thrombus formation plays a primary role in potentiating acute cardiovascular events, and this study was undertaken to determine whether pulmonary exposure to PM alters hemostasis. PM was collected from the Chapel Hill, NC airshed and was administered to mice by intratracheal instillation at a dose previously shown to exacerbate myocardial ischemia-reperfusion injury. Twenty-four hours after exposure, an increase occurred in the number of circulating platelets and plasma concentrations of fibrinogen and soluble P-selectin. The concentration of tissue factor pathway inhibitor (TFPI) in plasma was decreased, whereas the plasma concentration of plasminogen activator inhibitor (PAI-1) was increased. Consistent with these observations, bleeding time from a tail-tip transection was shortened. These results provide evidence that PM exposure alters hemostasis in otherwise healthy animals and may thereby promote clot formation and impede clot resolution in susceptible individuals. The results also establish definite hemostatic endpoints that can be used to further investigate the effects of dose and particle characteristics on the toxicity of ambient particles.