Role of focal adhesion kinase in oocyte-follicle communication.
Role of focal adhesion kinase in oocyte-follicle communication.
复制标题
粘着斑激酶在卵母细胞-卵泡通讯中的作用。
DOI：
10.1002/mrd.22446
复制
发表时间：
2015
影响因子：
2.5
通讯作者：
Kinsey,WilliamH
中科院分区：
文献类型：
--
作者：
McGinnis,LyndaK;Kinsey,WilliamH
生殖细胞需要与相关的体细胞沟通才能正常配子发生，例如，卵母细胞通过旁分泌因子以及位于这两种细胞之间接触点的缝隙连接与颗粒细胞相互作用。本研究的目的是确定控制细胞与卵母细胞接触的机制，并确定卵母细胞积极参与这种联系的程度。富含脯氨酸的酪氨酸激酶2(PTK2)是一种粘着斑激酶，在卵母细胞与周围颗粒细胞的跨带细胞突起接触处被激活。为了确定卵母细胞来源的PTK2信号在卵母细胞-卵泡通讯中的功能意义，通过将Ptk2-CAT-eGFP小鼠与Zp3-Creline配对的育种策略，获得了卵母细胞特异性的Ptk2基因敲除。由于Ptk2基因缺失的小鼠不会发育到出生，这为确定PTK2在卵母细胞-卵泡通讯中的作用提供了第一次机会。去除发育中的卵母细胞内的Ptk2会导致受精率降低，幼崽数量减少，囊胚形成率降低，每个囊胚的细胞数减少。含有Ptk2缺失卵母细胞的卵泡表现为卵母细胞直径变小，卵母细胞表面连接蛋白37和43焦点减少，卵母细胞与颗粒细胞之间的染料偶联减弱。这些发现与一个模型一致，在该模型中，PTK2在建立或维持卵母细胞-颗粒细胞接触中发挥关键作用，这种接触对于颗粒细胞和卵细胞之间的缝隙连接介导的通讯至关重要。是Reprod。戴夫。2015年，82：90-102。©2014 Wiley期刊，Inc.
Germ cells require communication with associated somatic cells for normal gametogenesis, as exemplified by an oocyte that interacts with granulosa cells via paracrine factors as well as gap junctions located at sites of contact between these two cell types. The objective of the present study was to define the mechanisms by which cell‐cell contact with the oocyte is controlled and to determine the extent that the oocyte actively participates in this association. Proline‐rich tyrosine kinase 2 (PTK2), a focal adhesion kinase, was found to be activated at sites of contact between the oocyte and trans‐zonal cell processes from the surrounding granulosa cells. In order to determine the functional significance of oocyte‐derived PTK2 signaling in oocyte‐follicle communication, an oocyte‐specificPtk2knockout was produced through a breeding strategy pairing a floxedPtk2‐CAT‐eGFPmouse with theZp3‐Creline. SincePtk2‐null mice never develop to birth, this represents the first opportunity to define the role of PTK2 in oocyte‐follicle communication. Ablation ofPtk2within the developing oocyte resulted in lower fertility with reduced numbers of pups, lower rates of blastocyst formation, and reduced cell numbers per blastocyst. Follicles containingPtk2‐null oocytes exhibited reduced oocyte diameter, reduced numbers of connexin 37 and 43 foci at the oocyte surface, and impaired dye coupling between oocyte and granulosa cells. These findings are consistent with a model in which PTK2 plays a critical role in establishing or maintaining oocyte‐granulosa cell contacts that are essential for gap junction‐mediated communication between granulosa cells and the oocyte.Mol. Reprod. Dev. 82: 90–102, 2015. © 2014 Wiley Periodicals, Inc.