Changes in Heat Shock Protein 70 and Ubiquitin mRNA Levels in C1300 N2A Mouse Neuroblastoma Cells Following Treatment with Iron
Changes in Heat Shock Protein 70 and Ubiquitin mRNA Levels in C1300 N2A Mouse Neuroblastoma Cells Following Treatment with Iron
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铁处理后 C1300 N2A 小鼠神经母细胞瘤细胞中热激蛋白 70 和泛素 mRNA 水平的变化
DOI：
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发表时间：
1993
影响因子：
4.7
通讯作者：
Sian M. Thomas
中科院分区：
文献类型：
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作者：
J. Uney;B. Anderton;Sian M. Thomas
摘要：我们已经表明，在热休克（42.5°C持续30分钟）后，小鼠来源的C1300 N2 A神经母细胞瘤细胞含有编码可诱导形式的热休克蛋白70和泛素的mRNA水平增加。用铁孵育C1300细胞还诱导编码相同两种蛋白质的mRNA含量升高，这两种蛋白质可被α-生育酚和去铁胺阻断。铁显示出在分化的C1300 N2 A培养物中增加线粒体和溶酶体活性，如3-（4，5-二甲基噻唑-2-基）-2，5-二苯基溴化四唑和中性红细胞毒性测定所示。通过乳酸脱氢酶释放试验评估，这些反应最初与任何活力丧失无关。这些结果表明，在神经细胞中，有细胞保护性热休克蛋白的产生，以响应铁介导的细胞损伤，可能涉及自由基的产生。在人类神经退行性疾病，特别是帕金森病中，脆弱神经元的明显应激反应可能是由退化神经元中铁介导的自由基产生引起的，从而研究了特别感兴趣的神经元细胞中自由基诱导反应的机制。
Abstract: We have shown that following heat shock (42.5°C for 30 min), mouse‐derived C1300 N2A neuroblastoma cells contain increased levels of mRNA coding for the inducible form of heat shock protein 70 and for ubiquitin. Incubation of C1300 cells with iron also induces an elevation in content of mRNAs coding for the same two proteins that can be blocked by α‐tocopherol and desferrioxamine. Iron was shown to increase mitochondrial and lysosomal activities in differentiated C1300 N2A cultures, as shown by the 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide and neutral red cytotoxicity assays. These responses were not initially associated with any loss of viability, as assessed by the lactate dehydrogenase release assay. These results suggest that there is production of cytoprotective heat shock proteins in response to iron‐mediated cell damage, probably involving free radical generation, in neural cells. The apparent stress response of vulnerable neurones in human neurodegenerative diseases, particularly Parkinson's disease, may be induced by iron‐mediated free radical production in degenerating neurones, making investigation of the mechanism of free radical‐induced responses in neuronal cells of special interest.
中国仓鼠卵巢细胞中热诱导的蛋白质去磷酸化。
DOI：
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发表时间：
1990
期刊：
Biomedica biochimica acta
影响因子：
--
作者：
Henle,KJ;Norris,JS;Lumpkin,CK
通讯作者：
Lumpkin,CK