Mucus Blockade of Lamellar Water Channels in Yellowtail Exposed to Chattonella marina
Mucus Blockade of Lamellar Water Channels in Yellowtail Exposed to Chattonella marina
复制标题
暴露于查托氏菌码头的黄尾鱼层状水道的粘液封锁
DOI：
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
T. Oda
中科院分区：
文献类型：
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作者：
Y. Hishida;A. Ishimatsu;T. Oda
(每次2小时)在阿尔辛无蓝缓冲液中，并在分级的乙醇系列中脱水。最后，样品在丙酮中浸泡两次，每次2分钟，风干。用相同的两种方法加工4条对照鱼。样品用奥林巴斯SZH-111体视显微镜观察。在第一种方法中，如果不在80%的乙醇中浸泡一夜，鳃上完全被盐晶体覆盖。而在第二种方法中，如果直接从液氮转移，样品将在戊二醛溶液中结冰。从进口侧观察，对照鱼的板层间通道清晰可见(图3)。IA、C)。与之形成鲜明对比的是，在暴露于扁桃体的鱼中，这些通道几乎完全被一种无定形的粘液样物质所覆盖(图1B，D)。覆盖在薄片上的层很薄，只在入口侧观察到
(each for 2 h) in Alcian blue-free buffer, and dehydrated in a graded series of ethanol. Finally, samples were immersed in acetone twice, each for 2 min, and air-dried. Four con trol fish were processed in the same two ways. Samples were examined with a stereomicroscope (Olympus SZH -111). With the first method, gills were totally covered by salt crystals if not immersed in 80% ethanol overnight. While in the second method, the samples would have been iced over in glutaraldehyde solution, if directly transferred from liquid nitrogen . The interlamellar channels of the control fish were clearly visible when viewed from the inlet side (Fig. IA, C). In sharp contrast, these channels were almost completely cov ered by an amorphous, mucus-like substance in the Chat tonella-exposed fish (Fig. 1B, D). The layer covering over the lamellae was thin, and only observed on the inlet side