Evidence for alternative splicing of MADS-box transcripts in developing cotton fibre cells
Evidence for alternative splicing of MADS-box transcripts in developing cotton fibre cells
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DOI：
10.1007/s00438-007-0297-y
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发表时间：
2008-01-01
影响因子：
3.1
通讯作者：
Orford, Sharon J.
中科院分区：
文献类型：
--
作者：
Lightfoot, Damien J.;Malone, Katharine M.;Orford, Sharon J.
MADS盒基因家族编码的转录因子在植物发育的各个方面具有广泛的作用。在这项研究中，4个棉花MADS盒cDNA克隆的II型（MIKC）类分离，系统发育分析表明，棉花序列的AGAMOUS亚类。相应的转录本在发育中的棉纤维细胞以及整个胚珠和花组织中检测到，在茎、叶和根中差异表达。逆转录PCR显示广泛的选择性剪接的反应之一，和11个不同的内含子/外显子组成和长度的mRNA进行了表征。转录本之间的序列差异表明它们不能来自相同的前mRNA，并且测序的转录本库来自两个不同的MADS盒基因。几种可变剪接转录本可能编码具有改变的K结构域和/或C末端区域的蛋白质，并且变体蛋白质可能具有改变的细胞作用。这项工作是第一个描述MADS盒基因在拉长棉纤维中的表达的工作，并为越来越多的证据增加了MADS盒和其他基因表达中选择性剪接的普遍性。
The MADS-box family of genes encodes transcription factors that have widely ranging roles in diverse aspects of plant development. In this study, four cotton MADS-box cDNA clones of the type II (MIKC) class were isolated, with phylogenetic analysis indicating that the cotton sequences are of the AGAMOUS subclass. The corresponding transcripts were detected in developing cotton fibre cells as well as in whole ovule and flower tissue, with differential expression in stems, leaves and roots. Reverse transcription PCR showed extensive alternative splicing in one of the reactions, and 11 mRNAs of different intron/exon composition and length were characterised. Sequence differences between the transcripts indicated that they could not be derived from the same pre-mRNA and that the sequenced transcript pool was derived from two distinct MADS-box genes. Several of the alternatively spliced transcripts potentially encoded proteins with altered K-domains and/or C-terminal regions and the variant proteins may have altered cellular roles. This work is the first that describes MADS-box gene expression in elongating cotton fibres and adds to a growing body of evidence for the prevalence of alternative splicing in the expression of MADS-box and other genes.