Characterization of the Sulfolobus host-SSV2 virus interaction
Characterization of the Sulfolobus host-SSV2 virus interaction
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DOI：
10.1007/s00792-006-0017-2
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发表时间：
2006-12-01
期刊：
影响因子：
2.9
通讯作者：
She, Qunxin
中科院分区：
文献类型：
--
作者：
Contursi, Patrizia;Jensen, Susanne;She, Qunxin
Sulfolobus纺锤体病毒SSV2编码一种酪氨酸整合酶，可促进宿主染色体中前病毒的形成。与序列分析中的预测一致，整合发生在tRNA(Gly)(CCC)基因的下游半部分。本文报道了SSV2在自然寄主Sulfolobus islandicus REY15/4和外来寄主Sulfolobus solfararicus中的比较研究结果，并为两种寄主SSV2生活史的不同调控提供了证据。事实上，虽然SSV2复制的显著诱导发生在自然宿主REY15/4的生长过程中，但SSV2 DNA的细胞含量在整个外来宿主的孵化过程中保持在相当低的水平。前一种情况下上体DNA的积累不能追溯到包装活性的降低，因为在培养基中同时增加了病毒滴度。此外，SSV2与其自然宿主的相互作用具有抑制宿主生长和诱导病毒DNA复制的并存特征。当用与自然宿主关系密切的岛链球菌REY15A研究这种病毒与宿主的相互作用时，发现SSV2复制过程的诱导方式与自然宿主REY15/4相同。
The Sulfolobus spindle virus, SSV2, encodes a tyrosine integrase which furthers provirus formation in host chromosomes. Consistently with the prediction made during sequence analysis, integration was found to occur in the downstream half of the tRNA(Gly) (CCC) gene. In this paper we report the findings of a comparative study of SSV2 physiology in the natural host, Sulfolobus islandicus REY15/4, versus the foreign host, Sulfolobus solfataricus, and provide evidence of differently regulated SSV2 life cycles in the two hosts. In fact, whereas a significant induction of SSV2 replication takes place during the growth of the natural host REY15/4, the cellular content of SSV2 DNA remains fairly low throughout the incubation of the foreign host. The accumulation of episomal DNA in the former case cannot be traced to decreased packaging activity because of a simultaneous increase in the virus titre in the medium. In addition, the interaction between SSV2 and its natural host is characterized by the concurrence of host growth inhibition and the induction of viral DNA replication. When this virus-host interaction was investigated using S. islandicus REY15A, a strain which is closely related to the natural host, it was found that the SSV2 replication process was induced in the same way as in the natural host REY15/4.