Flagellins, but Not Endoflagellar Sheath Proteins, of Treponema pallidum and of Pathogen-Related Oral Spirochetes Are Glycosylated
Flagellins, but Not Endoflagellar Sheath Proteins, of Treponema pallidum and of Pathogen-Related Oral Spirochetes Are Glycosylated
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梅毒螺旋体和病原体相关口腔螺旋体的鞭毛蛋白（但不是内鞭毛鞘蛋白）被糖基化
DOI：
10.1128/iai.66.12.5751-5754.1998
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发表时间：
1998
影响因子：
3.1
通讯作者：
C. Wyss
中科院分区：
文献类型：
--
作者：
C. Wyss
摘要梅毒螺旋体及其7种口腔密螺旋体的标准株和参考株鞭毛核心蛋白（FlaBs）存在糖基化。在几个非运动型菌株的口腔密螺旋体，FlaBs是检测不到的抗体和聚糖染色。相反，T. Vincenti相关菌株RitzA，OMZ 305 A，缺乏鞭毛鞘蛋白（FlaA）和野生型的两条聚糖染色FlaB带，但抗体标记显示了一条相对分子量较低的新FlaB带。一约38-分离的T.用单克隆抗体（MAb）H9-2在Western印迹上鉴定vincentii OMZ 800为FlaA，所述单克隆抗体（MAb）H9-2特异性标记T.苍白球聚糖和H9-2标记模式与T.在T. G7201培养基中分离的10株T. vincentii和两个T. Vincentii相关分类群。这四组被鉴定为可培养的病原体（T.苍白球）相关的口腔螺旋体，如用MAb H9-2标记所定义。H9-2单抗在食淀粉木霉、食淀粉木霉和食淀粉木霉中均未检测到反应性成分。denticola，齿齿叶蜂T. maltophilum、嗜麦芽窄食单胞菌T. pectinovorum和T. socranskii。
ABSTRACT Glycosylation of the flagellar core proteins (FlaBs) was detected in Treponema pallidum Nichols and in the type or reference strains of seven oral Treponema species. In several nonmotile strains of oral treponemes, the FlaBs were undetectable by both antibody and glycan staining. In contrast, a spontaneous low-motility variant of T. vincenti£i-related strain RitzA, OMZ 305A, lacked the flagellar sheath protein (FlaA) and the two glycan-staining FlaB bands of the wild type, but antibody labeling revealed a novel FlaB band with a lower relative molecular weight. A ca. 38-kDa component of isolated endoflagella of T. vincentii OMZ 800 was identified on Western blots as FlaA by monoclonal antibody (MAb) H9-2, which specifically labels the 37-kDa FlaA protein of T. pallidum. Glycan and H9-2 labeling patterns similar to those of T. pallidum were observed in whole-cell extracts of T. medium G7201 and of 10 strains classified as T. vincentii and as two T. vincentii-related taxons. These four groups were thus identified as cultivable pathogen (T. pallidum)-related oral spirochetes as defined by labeling with MAb H9-2. No H9-2 MAb-reactive component could be detected inT. amylovorum, T. denticola, T. maltophilum, T. pectinovorum, and the three subspecies of T. socranskii.
DOI：
--
发表时间：
1985
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Lukehart,SA;Tam,MR;Hom,J;Baker-Zander,SA;Holmes,KK;Nowinski,RC
通讯作者：
Nowinski,RC